{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:32:49Z","timestamp":1792045969207,"version":"4.4.0"},"reference-count":82,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,4,27]],"date-time":"2025-04-27T00:00:00Z","timestamp":1745712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objective<\/jats:title>\n                    <jats:p>Proinflammatory cytokines and enzymes responsible for tissue destruction are important in the development of periodontitis. This study compared salivary concentrations of interleukin\u20101 beta (IL\u20101\u03b2), matrix metalloproteinases (MMP\u20108), and (MMP\u20109) in individuals with and without periodontitis to evaluate their diagnostic utility as potential biomarkers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>A comprehensive search was performed across PubMed, Scopus, ScienceDirect, and Google Scholar, supplemented by manual searches in relevant journals up to January 2024. Eligibility criteria focused on human studies with defined diagnostic criteria for periodontitis and saliva samples analyzed for IL\u20101\u03b2, MMP\u20108, and MMP\u20109. Data were extracted to compare salivary levels of these markers between periodontitis patients and healthy controls. The Joanna Briggs Institute tool was used to evaluate the risk of bias and quality of the included studies. Statistical analysis employed a random effects model to calculate standardized mean differences and assess heterogeneity and publication bias.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The search yielded 122 articles, with 27 meeting the inclusion criteria. Fifteen percent of these studies presented a moderate risk of bias, while the remaining 85% exhibited a low risk of bias. The meta\u2010analyses indicated significantly higher levels of IL\u20101\u03b2, MMP\u20108, and MMP\u20109 in the saliva of subjects with periodontitis compared to healthy individuals: IL\u20101\u03b2: Standardized Mean Difference (SMD)\u2009=\u2009163.29 (95% CI\u2009=\u2009104.64\u2013221.95),\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001; MMP\u20108: SMD\u2009=\u2009282.22 (95% CI\u2009=\u2009209.68\u2013354.77),\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001; MMP\u20109: SMD\u2009=\u2009311.85 (95% CI\u2009=\u2009179.64\u2013444.05),\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Elevated salivary levels of IL\u20101\u03b2, MMP\u20108, and MMP\u20109 are linked to periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70040","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T02:05:54Z","timestamp":1745805954000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Levels of IL\u20101\u03b2,\n                    <scp>MMP<\/scp>\n                    \u20108, and\n                    <scp>MMP<\/scp>\n                    \u20109 in the Saliva of Subjects With Periodontitis: A Systematic Review and Meta\u2010Analysis"],"prefix":"10.1002","volume":"39","author":[{"given":"Mario Alberto","family":"Alarc\u00f3n\u2010S\u00e1nchez","sequence":"first","affiliation":[{"name":"Doctor of Science in Molecular Biology in Medicine Program, University Center of Health Sciences University of Guadalajara (CUCS\u2010UdeG)  Guadalajara Jalisco Mexico"},{"name":"Institute of Research in Dentistry, Department of Integral Dental Clinics, University Center of Health Sciences University of Guadalajara (CUCS\u2010UdeG)  Guadalajara Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Rodr\u00edguez\u2010Monta\u00f1o","sequence":"additional","affiliation":[{"name":"Institute of Research in Dentistry, Department of Integral Dental Clinics, University Center of Health Sciences University of Guadalajara (CUCS\u2010UdeG)  Guadalajara Jalisco Mexico"},{"name":"Department of Health and Illness as an Individual and Collective Process, University Center of Tlajomulco University of Guadalajara (CUTLAJO\u2010UdeG)  Tlajomulco de Zu\u00f1iga Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6963-8289","authenticated-orcid":false,"given":"Seyed Ali","family":"Mosaddad","sequence":"additional","affiliation":[{"name":"Department of Research Analytics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences Saveetha University  Chennai India"},{"name":"Department of Conservative Dentistry and Bucofacial Prosthesis, Faculty of Odontology Complutense University of Madrid  Madrid Spain"},{"name":"Department of Prosthodontics, School of Dentistry Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8329-3205","authenticated-orcid":false,"given":"Artak","family":"Heboyan","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Faculty of Stomatology Yerevan State Medical University after Mkhitar Heratsi  Yerevan Armenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,27]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12030685"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/476068"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13769"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12530"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.23963"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12270"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/children11020238"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12070865"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883\u2010020\u201000848\u20106"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/epirev\/mxx006"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010020\u201000488\u20106"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/jpm13020166"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.1902840142"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032\u2010016\u20100863\u20104"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242417328"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368\u2010019\u20100064\u2010z"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232113242"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031806"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.021908.132612"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2014.12.009"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2018.73345"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2007.01.011"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17144923"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1807"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2016.07.013"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2004.12.009"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.080021"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12159"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18020440"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cre2.865"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jobcr.2021.10.003"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/3664516"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201085120\u2010w"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903\u2010021\u201001600\u20105"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12781"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13349"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12544"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2017.10.004"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2017.170081"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/cre2.76"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12450"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2017.170023"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12521"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2016.160046"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11684\u2010014\u20100347\u2010x"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.5114\/ceji.2014.45948"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875\u2010012\u20109771\u20103"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12020"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2010.10.021"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10517\u2010011\u20101498\u20102"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.100472"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010051X.2010.01563.x"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.080480"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100765.2008.01119.x"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.50.53"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.2006.0181"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n71"},{"key":"e_1_2_12_59_1","first-page":"217","volume-title":"Systematic Reviews of Etiology and Risk","author":"Moola S.","year":"2017"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.781378"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12031003"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.25876"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12850"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.110616"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.5937\/bjdm20220824\u2010004"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/clr.12713"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.23736\/S2724\u20106329.23.04778\u20102"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874210601307010088"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25052721"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11081503"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10020061"},{"key":"e_1_2_12_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12423"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100765.2011.01432.x"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010051x.2005.00665.x"},{"key":"e_1_2_12_75_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.103200"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.1590\/1807\u20103107bor\u20102018.vol32.0114"},{"key":"e_1_2_12_77_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010051x.2009.01488.x"},{"key":"e_1_2_12_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100765.2004.00720.x"},{"key":"e_1_2_12_79_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509339487"},{"key":"e_1_2_12_80_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974\u20108520.141947"},{"key":"e_1_2_12_81_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12495"},{"key":"e_1_2_12_82_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000009642"},{"key":"e_1_2_12_83_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11061514"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70040","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T06:51:54Z","timestamp":1747723914000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.70040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,27]]},"references-count":82,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/jcla.70040"],"URL":"https:\/\/doi.org\/10.1002\/jcla.70040","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,27]]},"assertion":[{"value":"2025-01-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70040"}}