{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T19:30:53Z","timestamp":1767900653883,"version":"3.49.0"},"reference-count":83,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Metabolic\u2010associated fatty liver disease (MAFLD), the most prevalent liver disorder globally, affects 20%\u201340% of the population and presents significant health challenges. Studies link MAFLD to male reproductive dysfunction, highlighting the need for effective interventions. This study investigates the impact of MAFLD on testicular function and evaluates the protective role of endurance training, with a focus on the GRP78\u2010IRE\u20101\u03b1\u2010ATF6 signaling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty\u2010four rats were allocated into two dietary groups (<jats:italic>n<\/jats:italic>\u2009=\u200922 each): a standard diet control group (C) and a high\u2010fat diet supplemented with fructose water group (FL). After 17\u2009weeks, histological analysis confirmed MAFLD development in the FL group, while the control group showed no pathological changes. Each dietary group was further subdivided into sedentary and endurance\u2010trained (T) subgroups (<jats:italic>n<\/jats:italic>\u2009=\u200910 per subgroup), resulting in four experimental groups: C, C\u2009+\u2009T, FL\u2009+\u2009T, and FL. At the end of the research, thyroid stimulating hormone (TSH), sex hormones (testosterone), tumor necrosis factor\u2010alpha (TNF\u2010a) as well as GRP78, IRE\u20101\u03b1, and AFT6 expression were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results indicated that MAFLD led to significant weight gain, disrupted serum levels of thyroid\u2010stimulating hormone, and impaired sex hormone profile. Additionally, MAFLD triggered ER stress, evidenced by dysregulated expression of genes in the GRP78\u2010IRE\u20101\u03b1\u2010ATF6 pathway. Remarkably, endurance training mitigated these adverse effects by normalizing hormonal profiles and restoring the expression of ER stress\u2010related genes. These findings highlight the critical role of ER stress in MAFLD\u2010induced male reproductive dysfunction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, the present study suggests endurance training as a promising treatment strategy for addressing MAFLD and its associated reproductive complications.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70042","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T11:50:57Z","timestamp":1746186657000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Endurance Training Alleviates Metabolic\u2010Associated Fatty\u2010Liver Disease (<scp>MAFLD<\/scp>)\u2010Related Testicular Impairments via Endoplasmic Reticulum Stress Regulation"],"prefix":"10.1002","volume":"39","author":[{"given":"Nastaran","family":"Teimouri","sequence":"first","affiliation":[{"name":"Clinical Research Development Center Imam Reza Hospital, Kermanshah University of Medical Sciences  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5786-0904","authenticated-orcid":false,"given":"Vahid","family":"Kazemizadeh","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Center Health Policy and Promotion Institute, Kermanshah University of Medical Sciences  Kermanshah Iran"},{"name":"Department of Sport Physiology Faculty of Sport Sciences, Razi University  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S407657"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21165927"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-019-0517-x"},{"issue":"8","key":"e_1_2_10_5_1","article-title":"Nonalcoholic Fatty Liver Disease and Hypothyroidism: What You Need to Know","volume":"14","author":"Bikeyeva V.","year":"2022","journal-title":"Cureus"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/20420188221145549"},{"issue":"4","key":"e_1_2_10_7_1","first-page":"841","article-title":"Investigating the Effect of 8\u2009Weeks of Endurance Training on the Levels of Inflammatory Markers (IL\u20106\/TNF\u2010\u03b1) and Some Metabolic Parameters in Male Rats With Nonalcoholic Fatty Liver Disease","volume":"14","author":"Kazemizadeh V.","year":"2024","journal-title":"Journal of Health and Safety at Work"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sxmr.2019.01.002"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/livers4040038"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.4788"},{"key":"e_1_2_10_11_1","unstructured":"S. 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