{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:06:33Z","timestamp":1785240393409,"version":"3.55.0"},"reference-count":98,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Various factors, including virulence determinants, biofilm formation, and antimicrobial resistance, contribute to the severity of infections caused by <jats:italic>Enterococcus<\/jats:italic> spp.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>\n<jats:italic>Enterococcus<\/jats:italic> isolates were obtained from hospitalized patients in Yazd, Iran, and identified using microbiological and molecular tests. High\u2010level resistance, biofilm formation, and the genes encoding virulence factors and resistance were investigated following standard methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>\n<jats:styled-content style=\"fixed-case\">\n<jats:italic>Enterococcus faecalis<\/jats:italic>\n<\/jats:styled-content> was the most prevalent species (60.7%), followed by <jats:styled-content style=\"fixed-case\">\n<jats:italic>Enterococcus faecium<\/jats:italic>\n<\/jats:styled-content> (30.4%). Linezolid was highly effective, with 94.6% of isolates being susceptible. However, more than 76% of isolates exhibited resistance to rifampin, erythromycin, tetracycline, and ciprofloxacin, and 94.6% were multidrug\u2010resistant (MDR). Additionally, 39.3% of the isolates were vancomycin\u2010resistant enterococci (VRE) with a MIC &gt;\u200932\u2009\u03bcg\/mL, and the <jats:italic>vanA<\/jats:italic> gene was detected in 35.7% of the isolates. High\u2010level resistance to gentamicin and streptomycin was seen in 60.7% and 50% of the isolates, respectively. The most prevalent aminoglycoside resistance gene was <jats:italic>aph<\/jats:italic>(<jats:italic>3<\/jats:italic>\u2032)<jats:italic>\u2010IIIa<\/jats:italic> (62.5%) followed by <jats:italic>ant<\/jats:italic>(<jats:italic>6<\/jats:italic>\u2032)<jats:italic>\u2010Ia<\/jats:italic> (58.9%), and <jats:italic>aac<\/jats:italic>(<jats:italic>6<\/jats:italic>\u2032)<jats:italic>\u2010Ie\u2010aph<\/jats:italic>(<jats:italic>2<\/jats:italic>\u2033)<jats:italic>\u2010Ia<\/jats:italic> (50%). The <jats:italic>ant<\/jats:italic>(<jats:italic>3<\/jats:italic>\u2033)<jats:italic>\u2010Ia<\/jats:italic> was found in only one isolate. Most of the isolates (87.5%) were biofilm producers, and the distribution of virulence\u2010encoding genes was as follows: <jats:italic>gelE<\/jats:italic> (66.1%), <jats:italic>efaA<\/jats:italic> (57.1%), <jats:italic>asa1<\/jats:italic> (51.8%), <jats:italic>esp<\/jats:italic> (25%), <jats:italic>cylA<\/jats:italic> (19.6%), and <jats:italic>hyl<\/jats:italic> (8.9%). Furthermore, the <jats:italic>ace<\/jats:italic> gene was present in 79.4% of <jats:styled-content style=\"fixed-case\">\n<jats:italic>E. faecalis<\/jats:italic>\n<\/jats:styled-content> isolates, while the <jats:italic>fnm<\/jats:italic> and <jats:italic>acm<\/jats:italic> genes were found in 76.5% and 23.5% of <jats:styled-content style=\"fixed-case\">\n<jats:italic>E. faecium<\/jats:italic>\n<\/jats:styled-content> isolates, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The study highlights the significant role of notable drug resistance and the widespread presence of virulence traits in the development of enterococcal infections.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70063","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T01:14:26Z","timestamp":1748394866000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Assessment of 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