{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T17:36:04Z","timestamp":1783186564583,"version":"3.54.6"},"reference-count":23,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pancreatic cancer remains one of the malignancies characterized by the most insidious onset and dire prognosis. Preliminary diagnostic biomarkers are essential for timely identification and improved prognosis. CircularRNA\u2010DLGAP4 (Circ\u2010DLGAP4) and microRNA\u20109 (miR\u20109) are intriguing, easily accessible genetic indicators for numerous diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To quantify serum expression levels of Circ\u2010DLGAP4 and miR\u20109 in pancreatic cancer patients and juxtapose these values with those of control persons to assess their potential as diagnostic biomarkers. It also aims to correlate their expression levels with the clinicopathological features of the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Quantitative analysis of serum samples collected from 40 pancreatic cancer patients and 40 control subjects with RT\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were statistically significant elevations in blood levels of Circ\u2010DLGAP4 and miR\u20109 in pancreatic cancer patients compared to healthy persons, with median (range) values of 8.2 (0.39\u201317.32), <jats:italic>p<\/jats:italic>\u2010value &lt;\u20090.001, and 9.5 (4.06\u201314.7), respectively. The blood level of Circ\u2010DLGAP4 exhibited a significant positive correlation with the serum concentration of miR\u20109 (<jats:italic>r<\/jats:italic>\u2009=\u20090.89, <jats:italic>p<\/jats:italic>\u2009=\u20090.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Circ\u2010DLGAP4 and miR\u20109 serve as diagnostic biomarkers for pancreatic cancer.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70076","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T12:18:12Z","timestamp":1753445892000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Serum Circ\u2010<scp>DLGAP4<\/scp> and <scp>miR<\/scp>\u20109 as Potential Biomarkers in Pancreatic Cancer"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4007-7868","authenticated-orcid":false,"given":"Marwa A.","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Fayoum University  Fayoum Egypt"},{"name":"Department of Biomedical Sciences, College of Medicine King Faisal University  Al\u2010Ahsa Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3031-3599","authenticated-orcid":false,"given":"Olfat G.","family":"Shaker","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman M.","family":"Ezzat","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Saad Zaghloul","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimaa Mohammed","family":"Elasmer","sequence":"additional","affiliation":[{"name":"Department of Clinical and Chemical Pathology, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa Mamdouh Ahmed","family":"Abdelhafeez","sequence":"additional","affiliation":[{"name":"Department of Clinical and Chemical Pathology, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Zaidan","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raghda Yahia Abu","family":"El\u2010Ela","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa N.","family":"AbdelHafez","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, National Cancer Institute Cairo University  Al Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman","family":"Fares","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad A.","family":"Al Abdulqader","sequence":"additional","affiliation":[{"name":"Department of Surgery, College of Medicine King Faisal University  Al Hofuf Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aeshah A.","family":"Awaji","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science University College of Taymaa, University of Tabuk  Tabuk Saudi 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