{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T03:16:23Z","timestamp":1767064583081,"version":"3.44.0"},"reference-count":23,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dengue infection (DI) is a significant global health concern, with severe cases leading to plasma leakage, organ failure, and shock. Identifying reliable biomarkers for early risk stratification is crucial for improving patient management.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This descriptive cross\u2010sectional study included 268 DI patients at 103 Military Hospital, Vietnam, from July 2022 to October 2023. Patients were classified into Non\u2010Severe DI and Severe DI groups per the 2009 WHO dengue guideline. High Fluorescent Lymphocyte Count (HFLC), a parameter from the Sysmex hematology analyzer, was analyzed. HFLC% represents the proportion of high fluorescent lymphocytes among total WBCs, while HFLC# is these cells' absolute count (G\/L). Statistical methods included Mann\u2013Whitney U, Kruskal\u2010Wallis, Chi\u2010square tests, ROC curve analysis, and binary logistic regression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HFLC% and HFLC# were significantly elevated in Severe DI compared to Non\u2010Severe DI (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). HFLC% negatively correlated with platelet count and positively with liver enzymes (AST, ALT), suggesting an association with severe complications. ROC analysis showed that HFLC# (AUC\u2009=\u20090.913, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001, cut\u2010off\u2009=\u20091.00\u2009G\/L) had 70.6% sensitivity and 90.8% specificity, while HFLC% (AUC\u2009=\u20090.833, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001, cut\u2010off\u2009=\u200913.15%) had 70.6% sensitivity and 80.5% specificity for predicting severe DI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Elevated HFLC at admission is strongly associated with severe DI, with HFLC# demonstrating excellent predictive accuracy. These findings suggest that HFLC is a promising biomarker for early identification of high\u2010risk dengue patients. Further large\u2010scale validation is required to confirm its clinical utility.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70083","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T13:00:14Z","timestamp":1753707614000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluating High Fluorescence Lymphocyte Count as a Predictor of Severe Dengue Infection"],"prefix":"10.1002","volume":"39","author":[{"given":"Anh Vu","family":"Hong","sequence":"first","affiliation":[{"name":"Military Hospital 103  Vietnam"},{"name":"Vietnam Military Medical University  Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0333-2612","authenticated-orcid":false,"given":"Kien","family":"Nguyen Trung","sequence":"additional","affiliation":[{"name":"Military Hospital 103  Vietnam"},{"name":"Vietnam Military Medical University  Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5174-4291","authenticated-orcid":false,"given":"Hanh","family":"Nguyen Thi Hien","sequence":"additional","affiliation":[{"name":"Military Hospital 103  Vietnam"},{"name":"Vietnam Military Medical University  Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1904-2101","authenticated-orcid":false,"given":"Hung","family":"Ta Viet","sequence":"additional","affiliation":[{"name":"Military Hospital 103  Vietnam"},{"name":"Vietnam Military Medical University  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