{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:27:37Z","timestamp":1788449257360,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Epstein Barr virus (EBV) is a ubiquitous virus that establishes latent infection in the host and plays a critical role in the development and prognosis of lymphomas. The presence of EBV\u2010DNA in peripheral blood is a widely used tumor marker. However, there is no consensus on the preferred blood compartment for EBV\u2010DNA testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively analyzed data from 256 lymphoma patients, including 21 with Hodgkin lymphoma, 96 with B\u2010cell lymphoma, and 139 with T\u2010cell or NK\/T\u2010cell lymphoma. Complete matched PBMC and plasma EBV\u2010DNA datasets were available, allowing correlation analysis and assessment of their respective clinical significance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Detectable pretreatment EBV\u2010DNA in either plasma or PBMCs was significantly associated with worse survival outcomes (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), with the worst prognosis observed in patients positive in both compartments. Longitudinal monitoring demonstrated that patients with negative EBV\u2010DNA or declining viral loads in PBMCs or plasma had significantly improved progression\u2010free survival compared to those with persistent positivity or increasing copy numbers (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Notably, changes in plasma EBV\u2010DNA levels showed higher accuracy than PBMC EBV\u2010DNA in reflecting treatment response. Multivariate analysis identified PBMC EBV\u2010DNA positivity as an independent prognostic factor for inferior OS (<jats:italic>p<\/jats:italic>\u2009=\u20090.031) and PFS (<jats:italic>p<\/jats:italic>\u2009=\u20090.003).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Both plasma and PBMC EBV\u2010DNA are valuable for prognostic evaluation in lymphoma patients. Plasma EBV\u2010DNA demonstrates superior utility for monitoring treatment response, whereas PBMC EBV\u2010DNA provides stronger prognostic information. Selection of the appropriate sample type should be tailored to the clinical context.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70092","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T12:25:29Z","timestamp":1755087929000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clinical Significance of Plasma and Peripheral Blood Mononuclear Cell <scp>EBV<\/scp>\u2010<scp>DNA<\/scp> in Lymphoma"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8272-3599","authenticated-orcid":false,"given":"Lu","family":"wang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guobing","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjun","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9067-7087","authenticated-orcid":false,"given":"Qingyun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2010.03705.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2019.00071"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.88"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2016.0271"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00770-5"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01718-16"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2014.12.030"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136476"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.37282"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(15)00002-2"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17543"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2015.1072766"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-09-454694"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2022.105320"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.594692"},{"key":"e_1_2_8_17_1","first-page":"475","article-title":"The Biology and Clinical Utility of EBV Monitoring in Blood","volume":"391","author":"Kanakry J.","year":"2015","journal-title":"Current Topics in Microbiology and Immunology"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.11.010"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-05-354142"},{"key":"e_1_2_8_20_1","first-page":"61","article-title":"The EBNA3 Family: Two Oncoproteins and a Tumour Suppressor That Are Central to the Biology of EBV in B Cells","volume":"391","author":"Allday M. 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