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Traditional diagnostics like microscopy and polymerase chain reaction (PCR) suffer from low sensitivity in low\u2010parasitemia cases or high costs ($230\/test), necessitating accessible, rapid assays for resource\u2010limited regions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A cross\u2010priming amplification combined with vertical flow visualization (CPA\u2010VF) assay, a straightforward molecular method targeting the 18S rRNA gene of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>B. microti<\/jats:italic>\n                      <\/jats:styled-content>\n                      , requires minimal equipment and facilitates rapid detection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Sensitivity\/Specificity: The CPA\u2010VF assay detected 2.56\u2009fg\/reaction (equivalent to 0.000004% parasitic red blood cells), with a sensitivity of 95.5% matching that of RT\u2010PCR but at a 60\u2010fold lower cost ($3.8\/test). It showed no cross\u2010reactivity with\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">B. duncani<\/jats:styled-content>\n                        ,\n                        <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      <\/jats:italic>\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>. divergens<\/jats:italic>\n                      <\/jats:styled-content>\n                      <jats:italic>, or Plasmodium<\/jats:italic>\n                      . Clinical Validation: Testing 49 positive samples (19 experimentally infected mice +30 artificially spiked) and 492 field samples, CPA\u2010VF demonstrated 95.5% sensitivity (95% CI: 88.2\u201398.7) and 95.5% specificity compared to nested PCR (nPCR). Intra\u2010assay coefficients of variation (CV) was 2.1%\u20137.2% and inter\u2010assay kappa coefficient was 0.94, confirming reliability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      CPA\u2010VF is a rapid, low\u2010cost ($3.8\/test), and instrument\u2010free diagnostic tool for\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>B. microti<\/jats:italic>\n                      <\/jats:styled-content>\n                      , particularly suitable for endemic regions where timely diagnosis reduces mortality risks from misdiagnosis as malaria. Its portability and visual readout address critical gaps in resource\u2010constrained settings.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70102","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T07:56:07Z","timestamp":1758268567000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Set of Rapid Diagnostic Tool for\n                    <i>Babesia microti<\/i>\n                    Infection"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8234-5056","authenticated-orcid":false,"given":"Yanan","family":"Bai","sequence":"first","affiliation":[{"name":"Laboratory Medicine Center The Second Hospital &amp; Clinical Medical School, Lanzhou University  Lanzhou Gansu P. R. 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