{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T15:03:32Z","timestamp":1773414212392,"version":"3.50.1"},"reference-count":10,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Discordant Vitamin B12 results can result from immunoassay interferences, potentially leading to unnecessary diagnostic procedures and misdiagnoses if not properly recognized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Case Presentation<\/jats:title>\n                    <jats:p>A 76\u2010year\u2010old male with a history of IgA nephropathy, hypertension, and other comorbidities presented with unexpectedly elevated total Vitamin B12 levels despite no supplement use and normal hematological and biochemical parameters. Polyethylene glycol (PEG) precipitation demonstrated markedly reduced recovery, indicating the presence of macro\u2010VitB12. Pretreatment with blocking reagents and exclusion of heterophilic antibodies confirmed the interference. The discrepancy observed between analytical platforms further supported this finding.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Vitamin B12 results should always be interpreted in conjunction with clinical findings. Polyethylene glycol (PEG) precipitation is a cost\u2010effective and accessible method for detecting macro\u2010VitB12, particularly in cases where analytical results are inconsistent with the clinical presentation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70143","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T08:05:35Z","timestamp":1770365135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discordant Vitamin\n                    <scp>B12<\/scp>\n                    Results in an\n                    <scp>IgA<\/scp>\n                    Nephropathy Patient"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6507-1227","authenticated-orcid":false,"given":"Oytun","family":"Portakal","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazende","family":"I\u015flak","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1245-1121","authenticated-orcid":false,"given":"Muhammed \u015e.","family":"Binici","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yahya","family":"B\u00fcy\u00fcka\u015fik","sequence":"additional","affiliation":[{"name":"Department of Hematology, Faculty of Medicine Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2021-1288"},{"issue":"3","key":"e_1_2_9_3_1","first-page":"436","article-title":"Polyethylene Glycol Precipitation as a Screening Method for Macroprolactinemia","volume":"45","author":"Fahie\u2010Wilson M. N.","year":"1999","journal-title":"Clinical Chemistry"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2023.117267"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.40"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2022.42.1.3"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43240-1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0004563214547779"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/34.1.27"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPPWHSB94FTOGU"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.5045\/br.2019.54.2.149"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70143","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.70143","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70143","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T11:03:43Z","timestamp":1773399823000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.70143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,6]]},"references-count":10,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/jcla.70143"],"URL":"https:\/\/doi.org\/10.1002\/jcla.70143","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,6]]},"assertion":[{"value":"2025-06-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70143"}}