{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T02:39:23Z","timestamp":1769740763899,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study aims to investigate the nature and extent of hemolyzed, icteric, or lipemic (HIL) interference on platelet aggregation (PA) using the TA\u20108\u2009V aggregometer (Diagnostica Stago, Asni\u00e8re sur Seine) equipped with a near infrared light source outside the typical absorbance range of HIL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Platelet\u2010Rich\u2010Plasma (PRP) samples were spiked with substances mimicking HIL interference: red blood cell hemolysate (RBCH; 0.3\u201320\u2009g\/L of hemoglobin), bilirubin (15\u2013400\u2009mg\/L), and a fat emulsion (Intralipid 20%: 0.5\u20133\u2009g\/L). Maximal intensity (MaxInt) and velocity (Vel) were recorded in the basal state and in response to ADP 5\u2009\u03bcmol\/L and collagen 2\u2009\u03bcg\/mL. RBCH solution was treated with apyrase 0.1\u2009U\/mL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Spontaneous aggregation appeared above 0.6\u2009g\/L RBCH and significantly intensified with increased RBCH concentrations. The addition of apyrase to RBCH prevented spontaneous aggregation regardless of the RBCH concentration and led to reduced interindividual variability. In response to ADP and collagen, MaxInt and Vel significantly decreased as apyrase\u2010treated RBCH concentrations increased. MaxInt and Vel in response to ADP or collagen were not affected by increasing concentrations of bilirubin. The presence of lipids significantly increases MaxInt in response to ADP or collagen starting at 0.5\u2009g\/L.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings suggest that PA testing using the TA\u20108\u2009V instrument is not significantly impacted by icterus and hyperlipidemia within the specified ranges in healthy individuals. However, it is crucial to reject grossly hemolysed samples (exceeding 0.6\u2009g\/L) to avoid interference with ADP released from red blood cells. Further research is needed to confirm these results in patients with platelet dysfunction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70151","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T07:31:20Z","timestamp":1765956680000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Spectral Interfering Substances on Light Transmission Platelet Aggregation Using Infrared Based Aggregometer"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9495-7820","authenticated-orcid":false,"given":"Manal","family":"Ibrahim\u2010Kosta","sequence":"first","affiliation":[{"name":"Laboratory of Hematology, Centre de R\u00e9f\u00e9rence Des Pathologies Plaquettaires, C2VN, INRAE, INSERM, Aix Marseille Universit\u00e9  Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ga\u00efa","family":"Zirka","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology, Centre de R\u00e9f\u00e9rence Des Pathologies Plaquettaires, C2VN, INRAE, INSERM, Aix Marseille Universit\u00e9  Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Carriere","sequence":"additional","affiliation":[{"name":"Diagnostica Stago  Asni\u00e8res\u2010sur\u2010Seine France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Ohlmann","sequence":"additional","affiliation":[{"name":"Diagnostica Stago  Asni\u00e8res\u2010sur\u2010Seine France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3927-5792","authenticated-orcid":false,"given":"Marie\u2010Christine","family":"Alessi","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology, Centre de R\u00e9f\u00e9rence Des Pathologies Plaquettaires, C2VN, INRAE, INSERM, Aix Marseille Universit\u00e9  Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/194927b0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.15.5.446"},{"key":"e_1_2_10_4_1","first-page":"86","article-title":"Macroscopic Studies of Platelet Aggregation. 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