{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:33:09Z","timestamp":1770751989052,"version":"3.50.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Bronchiectasis, a leading chronic airway disease, often worsens due to infections, making rapid pathogen detection crucial. This study aims to evaluate the diagnostic value of bronchoalveolar lavage fluid (BALF) metagenomic next\u2010generation sequencing (mNGS) in identifying pathogens in moderate\u2010to\u2010severe bronchiectasis and compare its advantages to conventional methods.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Fifty\u2010two hospitalized patients initially diagnosed with moderate\u2010to\u2010severe bronchiectasis at Foshan Hospital of Traditional Chinese Medicine from May 2022 to March 2024 were enrolled. Clinical data and BALF samples were collected and subjected to both mNGS and conventional pathogen detection methods. The differences and concordance in pathogen distribution between mNGS and conventional methods, as well as their diagnostic performance, were compared.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The positive detection rate of pathogens by mNGS was significantly higher than that by conventional methods (\n p<\/jats:italic>\n \u2009<\u20090.01). Both methods predominantly identified bacterial pathogens, with\n \n Pseudomonas aeruginosa<\/jats:italic>\n <\/jats:styled-content>\n being the most common bacterium and\n \n Aspergillus fumigatus<\/jats:italic>\n <\/jats:styled-content>\n the most frequent fungus. However, mNGS detected a broader range of pathogens and demonstrated superior sensitivity in identifying mixed infections (\n p<\/jats:italic>\n \u2009<\u20090.01). The sensitivity of mNGS was 66% higher than that of conventional methods (\n p<\/jats:italic>\n \u2009<\u20090.01), and the complete concordance rate between the two methods in double\u2010positive cases was 41.18%. Additionally, mNGS\u2010guided anti\u2010infection treatment significantly improves patient symptoms, reduces hospital stays, and lowers costs (\n p<\/jats:italic>\n \u2009<\u20090.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Compared with conventional methods, BALF mNGS demonstrates higher sensitivity, a greater positive detection rate, superior capability in identifying mixed infections, improved diagnostic performance, and a better guiding effect on anti\u2010infection treatment in moderate\u2010to\u2010severe bronchiectasis.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcla.70156","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T06:02:26Z","timestamp":1766988146000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Application Value of Bronchoalveolar Lavage Fluid Metagenomic Next\u2010Generation Sequencing in Moderate\u2010to\u2010Severe Bronchiectasis"],"prefix":"10.1002","volume":"40","author":[{"given":"Jiachun","family":"Li","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine Foshan Guangdong China"},{"name":"Department of Respiratory Medicine Foshan Hospital of Traditional Chinese Medicine Foshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3852-7739","authenticated-orcid":false,"given":"Qiuping","family":"Quan","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine Foshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junshan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine Foshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyun","family":"Jian","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine Foshan Guangdong China"},{"name":"Department of Respiratory Medicine Foshan Hospital of Traditional Chinese Medicine Foshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weijie","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine Foshan Guangdong China"},{"name":"Department of Respiratory Medicine Foshan Hospital of Traditional Chinese Medicine Foshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingmin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine Foshan Guangdong China"},{"name":"Department of Respiratory Medicine Foshan Hospital of Traditional Chinese Medicine Foshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongbin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine Foshan Guangdong China"},{"name":"Department of Respiratory Medicine Foshan Hospital of Traditional Chinese Medicine Foshan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,28]]},"reference":[{"issue":"4","key":"e_1_2_14_2_1","first-page":"311","article-title":"Expert Consensus on the Diagnosis and Treatment of Adult Bronchiectasis in China","volume":"44","author":"Chinese Thoracic Society Bronchiectasis Working Group","year":"2021","journal-title":"Zhonghua Jie He He Hu Xi Za Zhi"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(23)00093-0"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.33073\/pjm-2024-007"},{"issue":"8","key":"e_1_2_14_5_1","first-page":"578","article-title":"Expert Consensus on Pathogen Detection in Bronchoalveolar Lavage Fluid for Pulmonary Infectious Diseases (2017 Version)","volume":"40","author":"Chinese Medical Association Respiratory Branch","year":"2017","journal-title":"Zhonghua Jie He He Hu Xi Za Zhi"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(21)00277-0"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathmechdis-012418-012751"},{"issue":"5","key":"e_1_2_14_8_1","first-page":"557","article-title":"Detection Advantages of Metagenomic Next\u2010Generation Sequencing in Bronchoalveolar Lavage Fluid of Severe Pneumonia","volume":"44","author":"Zhou X. L.","year":"2023","journal-title":"Journal of Wuhan University (Medical Sciences)"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1516"},{"issue":"2","key":"e_1_2_14_10_1","first-page":"151","article-title":"Expert Consensus on Metagenomic Diagnostic Technology in Acute and Critical Infections","volume":"28","author":"Metagenomic Analysis in Critical Infections Expert Consensus Group","year":"2019","journal-title":"Zhonghua Ji Zhen Yi Xue Za Zhi"},{"key":"e_1_2_14_11_1","first-page":"568","volume-title":"National Guidelines for Clinical Laboratory Testing Procedures [in Chinese]","author":"Shang H.","year":"2015"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201706-1097LE"},{"key":"e_1_2_14_13_1","unstructured":"L. L.Fang \u201cDiagnostic Value of Metagenomic Next\u2010Generation Sequencing of Bronchoalveolar Lavage Fluid in Lower Respiratory Tract Infections\u201d[in Chinese] (master's thesis Bengbu Medical College 2020 10\u201311)."},{"key":"e_1_2_14_14_1","unstructured":"C. C.He \u201cDiagnostic Value of Bronchoalveolar Lavage Fluid mNGS for Pathogens in Lower Respiratory Tract Infections\u201d[in Chinese] (master's thesis Dali University 2022 7\u201313)."},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18092011"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy693"},{"issue":"1","key":"e_1_2_14_17_1","first-page":"31","article-title":"Clinical Value of Bronchoalveolar Lavage Fluid Metagenomic Second\u2010Generation Sequencing Technology in Patients with Severe Pneumonia","volume":"38","author":"Chen Y. L.","year":"2022","journal-title":"Modern Medicine and Health"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2019.03.001"},{"issue":"11","key":"e_1_2_14_19_1","first-page":"98","article-title":"Comparison of Metagenomic Next\u2010Generation Sequencing and Traditional Laboratory Microbial Culture in the Etiological Diagnosis of Bronchiectasis","volume":"8","author":"Liu Q.","year":"2023","journal-title":"Doctor"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix881"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-024-02903-1"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70156","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.70156","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70156","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T13:40:38Z","timestamp":1770730838000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.70156"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,28]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/jcla.70156"],"URL":"https:\/\/doi.org\/10.1002\/jcla.70156","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,28]]},"assertion":[{"value":"2025-05-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70156"}}