{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:18:06Z","timestamp":1770751086741,"version":"3.50.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objectives<\/jats:title>\n To establish reference intervals of lymphocyte subpopulations in the healthy Chinese Han adult population and to explore the relationship between the lymphocyte subpopulations and routine health indicators.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We enrolled 120 healthy individuals aged between 22 and 60. Flow cytometry was utilized to assess lymphocyte subpopulations in peripheral blood samples. Variance analysis was conducted to determine reference intervals and to evaluate the effects of age and gender. Cluster analysis and correlation coefficient tests were employed to elucidate the relationships between lymphocyte subpopulations and routine health indicators.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Reference intervals of lymphocyte subpopulations among healthy adults were established. Significant differences between male and female were observed in the levels of central memory\n CD8<\/jats:styled-content>\n +<\/jats:sup>\n T cells (\n p<\/jats:italic>\n \u2009=\u20090.03), effector memory\n CD8<\/jats:styled-content>\n +<\/jats:sup>\n T cells (\n p<\/jats:italic>\n \u2009=\u20090.04), and Th2\u2010like T cells (\n p<\/jats:italic>\n \u2009=\u20090.01). An increase in the percentage of\n CD4<\/jats:styled-content>\n +<\/jats:sup>\n T cells (\n p<\/jats:italic>\n \u2009=\u20090.01) and\n HLADR<\/jats:styled-content>\n +<\/jats:sup>\n CD8<\/jats:styled-content>\n +<\/jats:sup>\n T cells (\n p<\/jats:italic>\n \u2009=\u20090.01), along with a decrease in na\u00efve\n CD8<\/jats:styled-content>\n +<\/jats:sup>\n T cells (\n p<\/jats:italic>\n \u2009=\u20090.01), were associated with increasing age. Cluster analysis indicated the potential of lymphocyte, including transitional B cells,\n CD3<\/jats:styled-content>\n +<\/jats:sup>\n T cells, na\u00efve\n CD4<\/jats:styled-content>\n +<\/jats:sup>\n T cells, na\u00efve B cells, and na\u00efve Treg, related to\n CA19<\/jats:styled-content>\n \u20109 and the percentage of lymphocyte in\n WBC<\/jats:styled-content>\n . Correlation tests showed the negative correlation between age and\n CD28<\/jats:styled-content>\n +<\/jats:sup>\n CD8<\/jats:styled-content>\n +<\/jats:sup>\n T cells (\n p<\/jats:italic>\n \u2009=\u20090.00) and the negative correlation between alcohol and na\u00efve B cells (\n p<\/jats:italic>\n \u2009=\u20090.02).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n This study not only established the normal reference intervals of lymphocyte subpopulations but also provided insights into the factors that may be related to these measurements, contributing valuable information for health management strategies.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcla.70159","type":"journal-article","created":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T07:41:02Z","timestamp":1768030862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reference Intervals for Lymphocyte Subpopulations in Healthy Chinese Han Adults: A Single\u2010Center Study"],"prefix":"10.1002","volume":"40","author":[{"given":"An\u2010Na","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Peking University First Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin\u2010Zi","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Peking University First Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Peking University First Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"You","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Peking University First Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Peking University First Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1743-9752","authenticated-orcid":false,"given":"Chen\u2010Xue","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Peking University First Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2011.10.005"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11604-008-0291-2"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ped.13701"},{"issue":"1","key":"e_1_2_12_5_1","first-page":"11","article-title":"Immune Status Monitoring of HIV\/AIDS Patients in Resource\u2010Limited Settings: A Review With an Emphasis on CD4+ T\u2010Lymphocyte Determination [J]","volume":"30","author":"Pattanapanyasat K.","year":"2012","journal-title":"Asian Pacific Journal of Allergy and Immunology"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.2741\/3487"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-010-0120-7"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.2009.00927.x"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.01.025"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2006.10.009"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.13010"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm-21-4031"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21162"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201970107"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.03.019"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.04.022"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0211207"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-022-01262-0"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ircmb.2018.06.002"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21323"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2014.14.6.289"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000337051"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00476-12"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2005.03.020"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2015.17"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.3.1191"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22788"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/84701"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2020.101435"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-018-0044-0"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030531"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004306"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.122.20001"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.122.18554"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajh\/hpx011"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-018-0399-6"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2022.215814"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-017-1985-z"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.131487"},{"issue":"8","key":"e_1_2_12_40_1","first-page":"1063","article-title":"Sleep and Antibody Response to Hepatitis B Vaccination [J]","volume":"35","author":"Prather A. A.","year":"2012","journal-title":"Sleep"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.5665\/sleep.4968"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2014.12.018"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00010.2018"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12877-021-02128-7"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0177-9"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.727959"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13613"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102595"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fragi.2024.1490302"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvae222"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641963.2025.2535328"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70159","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.70159","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70159","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T13:40:40Z","timestamp":1770730840000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.70159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,9]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/jcla.70159"],"URL":"https:\/\/doi.org\/10.1002\/jcla.70159","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,9]]},"assertion":[{"value":"2025-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70159"}}