{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T01:59:47Z","timestamp":1779933587877,"version":"3.53.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Janus Kinase 2 (JAK2) is a tyrosine kinase involved in cellular signalling. A point mutation in codon 617 of JAK2 (JAK2V617F) causes a gain\u2010of\u2010function effect that stimulates proliferation of myeloid cells. The aim of this study was to evaluate the Primerdesign quantitative allele\u2010specific amplification (Quasa) kit for the detection of JAK2V617F.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Specimens previously evaluated using real\u2010time PCR with melting curve analysis were analysed using the Quasa kit on the Bio\u2010Rad CFX 96 platform. Results of the two PCR tests were compared to evaluate the accuracy of the kit. For intra\u2010assay precision, samples were analysed in duplicate on the same run, and for inter\u2010assay variation, the same known samples were tested on three non\u2010consecutive days. The analytical sensitivity and quantitative analysis of the assay were determined using the World Health Organization (WHO) 1st International Reference Panel for Genomic JAK2V617F (United Kingdom Official Medicines Control Laboratory) and the United Kingdom National External Quality Assessment Service (UK NEQAS) results of their JAK2 p.val617phe (V617F) Mutation Status Programme.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Concordance of the qualitative test results between the test methods was 100%. Precision experiments confirmed repeatability and reproducibility. Analytical sensitivity was confirmed at 0.1%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The PrimerDesign Quasa kit for the detection of JAK2V617F is a sensitive and reliable assay that yields reproducible qualitative results. The kit is commercially available and has, in addition, the potential for quantitative analysis when samples are analysed in duplicate. This kit assay validation may be valuable to laboratories offering or interested in offering JAK2V617F mutational testing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70180","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T10:49:42Z","timestamp":1771498182000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Validation of the Primerdesign Quantitative Allele Specific Amplification Kit for the Detection of\n                    <scp>JAK2V617F<\/scp>\n                    Mutation"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7406-5307","authenticated-orcid":false,"given":"Deborah","family":"Vaz","sequence":"first","affiliation":[{"name":"National Health Laboratory Service \u2010 Tshwane Academic Division  Pretoria South Africa"},{"name":"Department of Haematology, Faculty of Health Sciences University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tshiphiri","family":"Netshidzivhani","sequence":"additional","affiliation":[{"name":"National Health Laboratory Service \u2010 Tshwane Academic Division  Pretoria South Africa"},{"name":"Department of Haematology, Faculty of Health Sciences University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5366-6744","authenticated-orcid":false,"given":"Johan","family":"Potgieter","sequence":"additional","affiliation":[{"name":"National Health Laboratory Service \u2010 Tshwane Academic Division  Pretoria South Africa"},{"name":"Department of Haematology, Faculty of Health Sciences University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00963"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-06-782037"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-10-695940"},{"key":"e_1_2_12_5_1","first-page":"431","article-title":"Chronic Myeloid Leukemia and Myeloproliferative Neoplasms","volume":"2016","author":"Mesa R. 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Genesig.com \u201d(2015)."},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0112-3"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2007.060083"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2017.06.004"},{"key":"e_1_2_12_23_1","first-page":"246","volume-title":"Laboratory Quality Management System Handbook","author":"Albetkova A.","year":"2011"},{"key":"e_1_2_12_24_1","volume-title":"Association for Molecular Pathology","author":"Bankowski M. 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