{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:37:39Z","timestamp":1791909459252,"version":"4.3.4"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005925","name":"Buddhist Tzu Chi Medical Foundation","doi-asserted-by":"publisher","award":["TCMMP112\u201001\u201003"],"award-info":[{"award-number":["TCMMP112\u201001\u201003"]}],"id":[{"id":"10.13039\/501100005925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005925","name":"Buddhist Tzu Chi Medical Foundation","doi-asserted-by":"publisher","award":["TCMF\u2010EP 110\u201002"],"award-info":[{"award-number":["TCMF\u2010EP 110\u201002"]}],"id":[{"id":"10.13039\/501100005925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005925","name":"Buddhist Tzu Chi Medical Foundation","doi-asserted-by":"publisher","award":["TCMF\u2010MP 113\u201001\u201002"],"award-info":[{"award-number":["TCMF\u2010MP 113\u201001\u201002"]}],"id":[{"id":"10.13039\/501100005925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022298","name":"Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation","doi-asserted-by":"publisher","award":["TCMF\u2010CM2\u2010112\u201005"],"award-info":[{"award-number":["TCMF\u2010CM2\u2010112\u201005"]}],"id":[{"id":"10.13039\/501100022298","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Beyond hemostasis and thrombosis, platelets are increasingly recognized as multifunctional cells involved in inflammation, vascular remodeling, and neurological diseases. Chronic cerebrovascular diseases impair cerebral blood flow and oxygen supply, leading to cognitive and motor dysfunction, where platelets may act as peripheral sensors reflecting neurovascular stress. This study investigated platelet activation profiles and platelet\u2013leukocyte interactions in acute intracerebral hemorrhage (aICH), subacute intracerebral hemorrhage (sICH), and chronic cerebrovascular diseases (CCD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Flow cytometry was used to assess platelet functions, including PAC\u20101 (activated GPIIb\/IIIa) and P\u2010selectin (CD62P), and platelet\u2013leukocyte aggregates, including myeloid\u2010platelet, lymphocyte\u2010platelet, and monocyte\u2010platelet in 20 CCD, 20 aICH, 30 sICH patients, and 48 healthy controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>PAC\u20101 expression was significantly reduced in aICH and sICH but elevated in CCD, indicating impaired integrin activation in acute and subacute stages and compensatory hyperreactivity in chronic disease. P\u2010selectin expression was markedly decreased in CCD under both ADP and TRAP stimulation, suggesting persistent \u03b1\u2010granule dysfunction in chronic conditions. Lymphocyte\u2013platelet aggregates, especially T\u2010cell interactions, were consistently diminished across all patient groups, reflecting disturbed platelet\u2013immune communication with T lymphocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Patients with cerebrovascular disorders show distinct platelet activation patterns and impaired immune crosstalk. Reduced P\u2010selectin expression and decreased lymphocyte\u2013platelet aggregation, especially T\u2010cell, indicate sustained platelet dysfunction and disrupted neuroinflammatory regulation. Flow cytometric assessment of these parameters provides valuable insight into neurovascular diseases at different stages and potential biomarkers for disease monitoring.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70192","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T05:35:09Z","timestamp":1773120909000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Flow Cytometric Profiling Reveals Platelet Dysfunction and Impaired Immune Communication in Acute and Chronic Cerebrovascular Disease"],"prefix":"10.1002","volume":"40","author":[{"given":"Chih\u2010Lung","family":"Shen","sequence":"first","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Internal Medicine Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien 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