{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T11:35:59Z","timestamp":1777030559161,"version":"3.51.4"},"reference-count":69,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82303994"],"award-info":[{"award-number":["82303994"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The psoriatic immune microenvironment (PIME) is central to psoriasis pathogenesis, yet its mechanistic drivers are incompletely defined. This study aimed to delineate immune cell infiltration patterns and identify pivotal disease\u2010related immune genes through a systematic analysis of the PIME.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We evaluated the infiltration levels of 28 immune cell subtypes in 11 psoriasis\u2010related microarray datasets using single\u2010sample gene set enrichment analysis (ssGSEA). Subsequent differential expression, consensus clustering, and weighted gene co\u2010expression network analysis (WGCNA) were employed to identify key genes. These findings were validated using human psoriatic tissue samples and an imiquimod\u2010induced murine psoriasis model to construct a predictive model termed IMscore.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our analysis identified five pivotal immune\u2010related differentially expressed genes (ImDEGs): CXCL8, CXCL9, CCL18, RGS1, and SAMSN1. A novel predictive model, IMscore, was constructed based on these ImDEGs to assess psoriasis risk. Furthermore, immune infiltration profiling and gene set enrichment analysis demonstrated that these ImDEGs are functionally associated with psoriasis\u2010related inflammatory pathways, validating the diagnostic utility of the IMscore framework.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These results provide new insights into the immunological mechanisms underlying psoriasis and establish a multi\u2010gene signature with potential for improving early diagnosis and therapeutic development.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70203","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T04:40:25Z","timestamp":1773981625000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Experimental Validation of an Immune Cell Infiltration Signature in Psoriasis: Translating Computational Modeling to In\u00a0Vivo Efficacy"],"prefix":"10.1002","volume":"40","author":[{"given":"Tingjin","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Quanzhou First Hospital Affiliated to Fujian Medical University  Quanzhou City Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Quanzhou Medical College  Quanzhou City Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Quanzhou First Hospital Affiliated to Fujian Medical University  Quanzhou City Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiujuan","family":"Wen","sequence":"additional","affiliation":[{"name":"Medical Research Center &amp; 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