{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T14:07:48Z","timestamp":1779026868468,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018553","name":"Science and Technology Plan Project of Wenzhou Municipality","doi-asserted-by":"publisher","award":["Y2020750"],"award-info":[{"award-number":["Y2020750"]}],"id":[{"id":"10.13039\/501100018553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Assess the application value of flow cytometry (FCM) for peripheral blood leukocyte (PBL) classification in acute leukemia (AL) screening and monitoring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Analyzed EDTA\u2010K2\u2010anticoagulated blood from 100 nonhematological patients, 42 newly diagnosed AL patients, and 50 posttreatment leukemia patients using FCM, blood cell analyzers (BCA), and manual microscopy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      FCM and BCA showed significant positive correlations in classifying and counting neutrophils, eosinophils, basophils, lymphocytes, and monocytes (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.994, 0.671, 0.946, 0.987, and 0.849, respectively;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). A significant positive correlation was observed between FCM and manual methods in counting blast cells (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.882,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). Using microscopy as the reference standard and a cutoff value of \u2265\u20091% blast cells, FCM demonstrated 100% sensitivity, 85.7% specificity, and 97.8% accuracy in detecting blast cells. The type of peripheral blood blast cells provided by FCM showed good consistency with the clinical diagnosis of leukemia (Kappa value\u2009=\u20090.834). Additionally, a positive correlation was found between the detection rates of bone marrow and peripheral blood blast cells using FCM in posttreatment patients with leukemia (\n                      <jats:italic>r<\/jats:italic>\n                      s\u2009=\u20090.860,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>FCM\u2010based PBL classification is precise and efficient for detecting blasts, providing rapid subtype identification. Its strong correlation with marrow findings underscores its clinical utility in AL screening and monitoring.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70206","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:08:26Z","timestamp":1773835706000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Application Value of Flow Cytometry\u2010Based Peripheral Blood Leukocyte Classification in Screening for Acute Leukemia and Posttreatment Monitoring"],"prefix":"10.1002","volume":"40","author":[{"given":"Penghua","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province The First Affiliated Hospital of Wenzhou Medical University  Wenzhou Zhejiang China"},{"name":"Zhejiang Key Laboratory of Intelligent Cancer Biomarker Discovery and Translation, First Affiliated Hospital Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianfeng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province The First Affiliated Hospital of Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangyang","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province The First Affiliated Hospital of Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province The First Affiliated Hospital of Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province The First Affiliated Hospital of Wenzhou Medical University  Wenzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11517-023-02955-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16360"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10432"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2001.069"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.08.030"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-011-1347-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11517-016-1590-x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10916-018-0962-1"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2009.014"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000186781.07221.F8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8588(01)00015-6"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3158"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20457"},{"issue":"3","key":"e_1_2_9_16_1","first-page":"131","article-title":"Reliable, Accurate Determination of the Leukocyte Differential of Leukopenic Samples by Using Hematoflow Method","volume":"31","author":"Jo Y.","year":"2011","journal-title":"Korean Journal of Laboratory Medicine"},{"key":"e_1_2_9_17_1","volume-title":"Haematolymphoid Tumours [Internet; Beta Version Ahead of Print]","author":"WHO Classification of Tumours Editorial Board","year":"2022"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2015.35.1.28"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.28475"},{"issue":"2","key":"e_1_2_9_20_1","first-page":"77","article-title":"Blood and Marrow Morphology in Acute Leukemia Patients Receiving Chemotherapy: A Photo\u2010Essay","volume":"49","author":"Shafer J. 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