{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T12:53:10Z","timestamp":1781527990802,"version":"3.54.1"},"reference-count":57,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["00023736"],"award-info":[{"award-number":["00023736"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NV18\u201008\u201000149"],"award-info":[{"award-number":["NV18\u201008\u201000149"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Thrombin activity plays a crucial role in hemostasis, and it is tightly regulated by a system of activators and inhibitors. Active, unregulated thrombin may initiate clotting without previous vessel injury, causing thrombosis. Thrombin bound to fibrin participates in fibrinogen transformation, while its inhibition by the naturally occurring heparin\u2013antithrombin complex is impeded. Bound thrombin activity has been detected on fibrin degradation products.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>D\u2010dimers were isolated using a commercial ELISA kit. Mass spectrometry was used to confirm the presence of thrombin on captured D\u2010dimers. D\u2010dimer\u2010bound thrombin activity was evaluated using specific substrate and inhibitors: hirudin\u2010PPACK and heparin\u2013antithrombin. Groups of 72 patients and 159 controls were analyzed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The activity of thrombin was assessed utilizing our system, demonstrating stability throughout the procedures. The thrombin concentration during fibrin polymerization was reflected in the thrombin activity levels detected on D\u2010dimers. Analysis of patient plasma samples indicated the feasibility of detecting low levels of active thrombin. Significant differences in D\u2010dimer\u2010bound thrombin activity were observed between subjects with chronic (controls) and acute conditions (patients).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We present a proof\u2010of\u2010concept method for the evaluation of thrombin activity on circulating D\u2010dimers that may aid in the diagnosis of thrombotic events and the monitoring of anticoagulant therapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70224","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:00:41Z","timestamp":1776769241000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Measurement of Active Thrombin Bound to Circulating D\u2010Dimers as a Sensitive Biomarker for Prothrombotic Conditions"],"prefix":"10.1002","volume":"40","author":[{"given":"Jana","family":"Stikarova","sequence":"first","affiliation":[{"name":"Department of Biochemistry Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leona","family":"Chrastinova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristyna","family":"Svobodova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Havlicek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Louzil","sequence":"additional","affiliation":[{"name":"Centre for Thrombosis and Hemostasis Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliska","family":"Ceznerova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alzbeta","family":"Hlavackova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Pastva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavla","family":"Pecherkova","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Bioinformatics Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Hrachovinova","sequence":"additional","affiliation":[{"name":"Laboratory for Disorders in Hemostasis Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Kucerka","sequence":"additional","affiliation":[{"name":"Department of Medicine First Faculty of Medicine, Charles University in Prague and Military University Hospital  Prague Czech Republic"},{"name":"Department of Military Internal Medicine and Military Hygiene, University of Defence  Hradec Kralove Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Kotlin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Soukup","sequence":"additional","affiliation":[{"name":"Inpatient Department Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Salaj","sequence":"additional","affiliation":[{"name":"Centre for Thrombosis and Hemostasis Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Maly","sequence":"additional","affiliation":[{"name":"Department of Medicine First Faculty of Medicine, Charles University in Prague and Military University Hospital  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4305-9718","authenticated-orcid":false,"given":"Jiri","family":"Suttnar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Hematology and Blood Transfusion  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(25)01917\u20108"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(20)32658\u20101"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/VHRM.S83718"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/clc.23160"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218414109"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-49674-0_13"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538\u20107836.2007.02500.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2008.113"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.410829"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH13\u201010\u20100811"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538\u20107836.2005.01363.x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2015.09.013"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052590"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.15647"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi2016519"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707710200"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb811"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102141.1999.01689.x"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0801082"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.97.6.544"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0049\u20103848(16)30106\u20102"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2008.01.001"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0369\u201012.2012"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538\u20107836.2009.03413.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.09.010"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2016.01.065"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201402244"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1109\/JSEN.2008.922725"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac801431m"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsanm.1c01350"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.4902908"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201007032"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201008\u2010300301"},{"key":"e_1_2_10_35_1","unstructured":"R Core Team \u201cR: A language and environment for statistical computing \u201d(2020) https:\/\/www.r\u2010project.org\/."},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.4103\/2229\u20105186.79345"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2014.04.055"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114723"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100038\u20101649856"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703137"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100038\u20101648947"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2004.04.014"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/1074248410395941"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102002\u201008\u20102527"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167\u20104838(89)90140\u20104"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100041\u20101739193"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V128.22.716.716"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102141.2011.08935.x"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10060923"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102009\u201005\u2010222380"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001250050607"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH08\u201011\u20100771"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.111.230631"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2022.40373"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100033\u20101356572"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqad138"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.124.320151"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.afjem.2019.05.003"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70224","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.70224","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70224","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T12:03:04Z","timestamp":1781524984000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.70224"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,21]]},"references-count":57,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/jcla.70224"],"URL":"https:\/\/doi.org\/10.1002\/jcla.70224","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,21]]},"assertion":[{"value":"2025-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70224"}}