{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:58:42Z","timestamp":1785373122820,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002738","name":"Assistance Publique - H\u00f4pitaux de Paris","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Current neonatal diagnostic tests for congenital toxoplasmosis (CT) have limited performance, which is particularly problematic in resource\u2010limited settings and where monitoring for toxoplasmosis during pregnancy is lacking. The TOXODIAG study (NCT03385499) aimed to detect specific IgGs as soon as they appear in pregnant women with acute infection with\n                      <jats:italic>Toxoplasma gondii<\/jats:italic>\n                      (\n                      <jats:italic>Tg<\/jats:italic>\n                      ) and their newborns suspected of infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Seventy pregnant women were included in five perinatal centers in Paris, France. They were divided into three groups based on their\n                      <jats:italic>Toxoplasma<\/jats:italic>\n                      seroconversion during pregnancy (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200920, group of interest) and their immune status at delivery with latent infection (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200923) or confirmed absence of infection (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200927, control groups). The enzyme\u2010linked immunospot (ELISPOT) method was used to detect B lymphocytes primed to produce IgG against the recombinant antigens\n                      <jats:italic>Tg<\/jats:italic>\n                      SAG1,\n                      <jats:italic>Tg<\/jats:italic>\n                      GRA7, and\n                      <jats:italic>Tg<\/jats:italic>\n                      AMA1.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Complete and validated ELISPOT results were obtained for 9 women in the SEROCO group, including 1 of the 5 CT cases resulting from pregnancy in this group.\n                      <jats:italic>Tg<\/jats:italic>\n                      \u2010specific IgG\u2010secreting cells were observed in mothers at the time of diagnosis of\n                      <jats:italic>Tg<\/jats:italic>\n                      seroconversion and delivery, but not in cord blood. A simplified version of the ELISPOT test, combining the three\n                      <jats:italic>Tg<\/jats:italic>\n                      antigens in a single plate well, reproduced the information provided by the antigens considered independently, with a positivity rate of 35% compared to a range of 6%\u201337%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The ELISPOT method could be useful for maternal screening, but for postnatal detection of\n                      <jats:italic>Tg<\/jats:italic>\n                      \u2010specific IgG\u2010secreting cells, it either requires further technical improvements or is not a suitable method.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70226","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T04:23:17Z","timestamp":1782966197000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of Specific\n                    <scp>IgG<\/scp>\n                    \u2010Secreting Cells in Congenital Toxoplasmosis: The\n                    <scp>TOXODIAG<\/scp>\n                    Study"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9982-594X","authenticated-orcid":false,"given":"F.","family":"Migot\u2010Nabias","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, IRD Inserm, MERIT  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Beldjoudi","sequence":"additional","affiliation":[{"name":"D\u00e9partement d'\u00e9pid\u00e9miologie et de recherche clinique AP\u2010HP, GH Paris Nord 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