{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T17:02:16Z","timestamp":1779814936720,"version":"3.53.1"},"reference-count":14,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Fluid Overload\u2010associated Large B\u2010Cell Lymphoma (FO\u2010LBCL) is one of the newly added entities in the WHO classification of Haematolymphoid Tumors 5th edition [1]. Formerly, these cases were classified as primary effusion lymphoma\u2010like (effusion) lymphoma (PEL\u2010like (effusion) lymphoma) or HHV8\u2010unrelated PEL\u2010like lymphoma. As knowledge grew regarding the differences in clinical presentation and prognosis of these entities, the need for a distinct classification became apparent.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>We describe a case of a 79\u2010year\u2010old man with acute dyspnea and cough due to massive pleural effusion, revealed by CT scan of the thorax. Further diagnostic evaluation with PET\u2010CT showed no detectable tumor mass apart from the pleural fluid. Pleural fluid cytology revealed numerous large atypical lymphoid cells. Flow cytometric immunophenotyping showed a predominant monoclonal CD19+\/CD20+ B\u2010cell population with light chain restriction and CD5 expression. Molecular analysis demonstrated partial clonality, and cytogenetics revealed a complex hyperdiploid karyotype. EBV in\u00a0situ hybridization and testing for HHV8 and HIV were negative. No underlying immunodeficiency or systemic disease was identified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Conclusion<\/jats:title>\n                    <jats:p>Based on these findings, diagnosis was most consistent with FO\u2010LBCL, but distinction from other HHV8\u2010negative effusion\u2010based large B\u2010cell lymphomas remains challenging due to overlapping features. The patient received R\u2010CHOP\u2010based immunochemotherapy and remains clinically stable without systemic progression. This case highlights the critical role of integrated cytologic, immunophenotypic, molecular, and virological analyses in establishing the correct classification of effusion\u2010based large B\u2010cell lymphomas and underscores the diagnostic challenges posed by this emerging entity. Accurate recognition of FO\u2010LBCL is essential, as its clinical behavior and therapeutic implications differ from other effusion\u2010based lymphomas.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70244","type":"journal-article","created":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T05:54:18Z","timestamp":1778306058000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolated Pleural Effusion as Presentation of a Large B\u2010Cell Lymphoma: A Case Report and Diagnostic Considerations"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6252-851X","authenticated-orcid":false,"given":"Van Vlierberghe","family":"Magalie","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Heilig Hart Hospital  Lier Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2399-895X","authenticated-orcid":false,"given":"Dewaide","family":"Rosina","sequence":"additional","affiliation":[{"name":"Department of Haematology Heilig Hart Hospital  Lier Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8357-7942","authenticated-orcid":false,"given":"Michaux","family":"Lucienne","sequence":"additional","affiliation":[{"name":"Center for Human Genetics University Hospitals Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5837-8506","authenticated-orcid":false,"given":"Develter","family":"Wim","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology Heilig Hart Hospital  Lier Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van Aelst","family":"Sophie","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Heilig Hart Hospital  Lier Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9637-5592","authenticated-orcid":false,"given":"Claerhout","family":"Helena","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Heilig Hart Hospital  Lier Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"e_1_2_12_2_1","volume-title":"WHO Classification of Tumours: Haematolymphoid Tumours, 5th Edn","author":"Campo E.","year":"2022"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/BLOODADVANCES.2020002293"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318267fabc"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015851"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2019.08.013"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102023\u2010174337"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000097460"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.4084\/MJHID.2022.020"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1145540"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010022\u201001091\u2010x"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428\u2010023\u201003599\u20102"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.12659\/AJCR.944268"},{"key":"e_1_2_12_14_1","volume-title":"StatPearls","author":"Padala S. 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