{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:58:44Z","timestamp":1785373124044,"version":"3.55.0"},"reference-count":25,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Hemoglobin British Columbia is a rare high\u2010oxygen\u2010affinity \u03b2\u2010globin variant caused by the HBB:c.304G>A substitution. Its detection is exceptionally uncommon, particularly in the Middle East, and may lead to diagnostic pitfalls when relying solely on hemoglobin separation techniques. Precise molecular characterization is therefore essential for accurate differentiation from other hemoglobinopathies and for expanding knowledge of their regional distribution.<\/jats:p>\n <\/jats:sec>\n \n Case Presentation<\/jats:title>\n We report a 25\u2010year\u2010old asymptomatic Iranian male who was incidentally identified during routine premarital screening due to the presence of an unexpected hemoglobin band. Comprehensive evaluation was performed using capillary zone electrophoresis (CZE), targeted analysis of common \u03b1\u2010globin deletions, full sequencing of the \u03b1\u2010 and \u03b2\u2010globin genes, and long\u2010range PCR for \u03b1\u2010gene triplications.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n CZE revealed a major abnormal hemoglobin fraction (73.2%), with HbA at 21.7% and HbA\n 2<\/jats:sub>\n at 5.1%. \u03b1\u2010Globin gene analysis showed no mutations. \u03b2\u2010Globin gene sequencing demonstrated compound heterozygosity for Hb British Columbia (HBB:c.304G>A) and the \u03b2\u2010thalassemia variant IVS\u2010I\u20106 (HBB:c.92+6T>C), a combination not previously reported. Despite the presence of two \u03b2\u2010globin variants, the patient exhibited normal hematologic and biochemical parameters and no clinical symptoms.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n This is the first documented case of co\u2010inheritance of Hb British Columbia and the IVS\u2010I\u20106 \u03b2\u2010thalassemia mutation in Iran. The case underscores the crucial role of molecular diagnostics in resolving atypical electrophoretic patterns, preventing misclassification of rare hemoglobin variants, and enriching the understanding of the hemoglobinopathy spectrum in diverse populations.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcla.70261","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T05:45:18Z","timestamp":1782193518000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Report of Co\u2010Inheritance of Hemoglobin British Columbia (\n HBB<\/scp>\n :c.\n 304G<\/scp>\n >A) and \u03b2\u2010Thalassemia\n IVS<\/scp>\n \u2010I\u20106 (\n HBB<\/scp>\n :c.92+\n 6T<\/scp>\n >C): Clinical Characterization of an Iranian Case"],"prefix":"10.1002","volume":"40","author":[{"given":"Kimia","family":"Fathalizade","sequence":"first","affiliation":[{"name":"Student Research Committee Bushehr University of Medical Sciences Bushehr Iran"},{"name":"Department of Hematology and Blood Transfusion, School of Allied Medicine Bushehr University of Medical Sciences Bushehr Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahsa Kiani","family":"Bigdeli","sequence":"additional","affiliation":[{"name":"Student Research Committee Bushehr University of Medical Sciences Bushehr Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Sajad","family":"Eghbali","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine Bushehr University of Medical Sciences Bushehr Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammadtaghi","family":"Ghiyasi","sequence":"additional","affiliation":[{"name":"Student Research Committee Bushehr University of Medical Sciences Bushehr Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roz","family":"Rastgoo","sequence":"additional","affiliation":[{"name":"Student Research Committee Bushehr University of Medical Sciences Bushehr Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4849-6706","authenticated-orcid":false,"given":"Mohammad Javad","family":"Mousavi","sequence":"additional","affiliation":[{"name":"Department of Immunology and Allergy, Faculty of Medicine Bushehr University of Medical Sciences Bushehr 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