{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:58:44Z","timestamp":1785373124052,"version":"3.55.0"},"reference-count":17,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n Exploring the application of urea dissociation treatment to reduce the false\u2010positive rate of ELISA for syphilis detection.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n From January 2023 to January 2024, eleven false\u2010positive syphilis samples were collected from Xi'an Regional Medical Laboratory Centre as the experimental group, five positive specimens as the control group, and five negative specimens as the control group. All specimens were divided into three groups: A, B, and C. They were treated with 6\u2009mol\/L urea for 10\u2009min, 6\u2009mol\/L urea for 20\u2009min, and 10\u2009mol\/L urea for 10\u2009min, respectively, and then processed according to the experimental procedures. Combined with the analysis of the TPPA and TP immunoblotting results, the Wilcoxon test was used to compare the S\/CO values of each group.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Specimens 2 and 3 still had no <\u20091.0\u2009S\/CO in group A, while B and C had <\u20091.0\u2009S\/CO after completion of the separation. Meanwhile, specimens 7 and 8 of the experimental group had S\/CO close to 1.0 after dissociation in all three groups. The positive control group had an S\/CO ratio of greater than 1.0 in all three groups. The negative control specimens, both before and after separation, had no effect. The differences in S\/CO values between experimental groups A, B, and C before and after dissociation were statistically significant (\n p<\/jats:italic>\n \u2009<\u20090.05). The values of TP are below the manufacturer's allowable imprecision requirements.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Urea dissociation by ELISA is effective in reducing the false\u2010positive rate of syphilis.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcla.70262","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T13:09:54Z","timestamp":1780578594000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Application of Urea Dissociation Treatment to Reduce the False\u2010Positive Rate of\n ELISA<\/scp>\n for Syphilis Detection"],"prefix":"10.1002","volume":"40","author":[{"given":"Jiao","family":"Wang","sequence":"first","affiliation":[{"name":"Xi'an Area Medical Laboratory Center Xi'an Shaanxi People's Republic of China"},{"name":"Shaanxi Lifegen Co., Ltd. Xi'an Shaanxi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Li","sequence":"additional","affiliation":[{"name":"Xi'an Area Medical Laboratory Center Xi'an Shaanxi People's Republic of China"},{"name":"Shaanxi Lifegen Co., Ltd. Xi'an Shaanxi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haodi","family":"Li","sequence":"additional","affiliation":[{"name":"Xi'an Area Medical Laboratory Center Xi'an Shaanxi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xucai","family":"Dong","sequence":"additional","affiliation":[{"name":"Xi'an Area Medical Laboratory Center Xi'an Shaanxi People's Republic of China"},{"name":"Wuhan Central Hospital of the Third Bureau of CECEC Wuhan Hubei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"issue":"8","key":"e_1_2_9_2_1","first-page":"1672","article-title":"Establishment of Measurement Threshold and Comparison of Results for Detecting Syphilis Spirochete\u2010Specific Antibodies by Siemens ADVIA Centaur XP Chemiluminescent Immunoassay Analyzer","volume":"36","author":"Wang X. 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P.","year":"2015","journal-title":"Journal of Clinical Haematology"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1413-86702008000200008"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.4.1568-1576.1992"},{"key":"e_1_2_9_17_1","first-page":"34","volume-title":"Immunology and Evolution of Infectious Disease","author":"Aaby P.","year":"2002"},{"issue":"7","key":"e_1_2_9_18_1","first-page":"736","article-title":"Evaluation of the Efficacy of the Urea Dissociation Method for Correcting False\u2010Positive Results of Syphilis Spirochete Antibody Detection by Chemiluminescence","volume":"36","author":"Gh P.","year":"2022","journal-title":"Journal of Chinese Practical Diagnosis and Therapy"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70262","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.70262","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:04:39Z","timestamp":1785369879000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.70262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,4]]},"references-count":17,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/jcla.70262"],"URL":"https:\/\/doi.org\/10.1002\/jcla.70262","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,4]]},"assertion":[{"value":"2025-02-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70262"}}