{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T14:50:43Z","timestamp":1787928643523,"version":"build-2784847793"},"reference-count":62,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Medicinal\u2013edible natural products like\n                      <jats:italic>Cordyceps militaris (C.militaris)<\/jats:italic>\n                      , a functional food rich in cordycepin (COR), are promising for health maintenance. Although COR possesses anti\u2010inflammatory and antioxidant properties, its protective efficacy and underlying mechanisms against acetaminophen (APAP)\u2010induced hepatotoxicity remain unclear. This study aimed to evaluate the influence of COR pretreatment on APAP\u2010induced acute liver injury and elucidate the underlying mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To this end, male C57BL\/6 mice were gavaged for 7\u2009days with NAC (N\u2010acetylcysteine) (200\u2009mg\/kg) or COR (50, 100, or 200\u2009mg\/kg), followed by APAP administration (400\u2009mg\/kg, gavage) to cause liver injury for 12\u2009h. Liver injury was evaluated by serum transaminase levels, histopathological analysis, inflammatory cytokine detection, oxidative stress markers, TUNEL staining, and RNA\u2010seq\u2010based transcriptomic analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>COR pretreatment markedly reduced serum transaminases and alleviated hepatic necrosis and inflammatory infiltration. RNA\u2010seq revealed significant APAP\u2010induced changes in genes associated with ER protein processing, HIF\u20101, and PPAR signaling, while COR reversed multiple key genes involved in oxidative stress, inflammation, and apoptosis. Consistent with transcriptomic findings, COR decreased pro\u2010inflammatory cytokines, mitigated oxidative damage, and inhibited hepatocyte apoptosis (TUNEL staining).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In conclusion, COR pretreatment attenuates APAP\u2010induced hepatotoxicity in mice by modulating ER proteostasis\u2010related pathways and coordinately influencing HIF\u20101 and PPAR\u2010associated signaling networks, thereby exerting anti\u2010inflammatory, antioxidant, and anti\u2010apoptotic effects. These findings support COR as a promising hepatoprotective candidate for APAP\u2010caused liver damage.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70266","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T15:33:42Z","timestamp":1779723222000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Edible Fungus Compound Cordycepin Protects Against Acetaminophen\u2010Induced Liver Injury"],"prefix":"10.1002","volume":"40","author":[{"given":"Chunjin","family":"Fu","sequence":"first","affiliation":[{"name":"State Key Laboratory for Quality Assurance and Sustainable Use of Dao\u2010di Herbs China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyu","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Assurance and Sustainable Use of Dao\u2010di Herbs China 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