{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T16:08:05Z","timestamp":1784304485537,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018715","name":"Faculty of Allied Health Sciences, Chulalongkorn University","doi-asserted-by":"publisher","award":["AHS02\/2567"],"award-info":[{"award-number":["AHS02\/2567"]}],"id":[{"id":"10.13039\/501100018715","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Hemoglobin Constant Spring (HbCS) and Hb Paks\u00e9 are clinically significant \u03b1\u2010globin variants prevalent in Southeast Asia that cause severe non\u2010deletional HbH disease when combined with \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      \u2010thalassemia deletions. Their inherent instability and low circulating levels (0.1%\u20131.0%) present analytical challenges, often yielding false negatives with conventional screening methods. This study aimed to evaluate Premier Resolution HPLC performance in detecting HbCS and Hb Paks\u00e9 vs. capillary electrophoresis (CE), and to characterize chromatographic patterns across \u03b1\u2010 and \u03b2\u2010globin genotype combinations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A two\u2010phase validation study was conducted. Phase 1 analyzed 616 samples (307 DNA\u2010confirmed HbCS\/Hb Paks\u00e9 cases) to characterize chromatographic patterns and compare Premier Resolution with CE. Phase 2 prospectively validated the system in 2032 consecutive individuals undergoing routine health screening. Molecular analysis served as the reference standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Premier Resolution HPLC demonstrated distinctive multi\u2010peak patterns spanning the S and C chromatographic windows for HbCS\/Hb Paks\u00e9, with quantification limited to homozygous and \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      \u2010thalassemia co\u2010inherited cases. It outperformed CE with 96.4% sensitivity, 97.1% specificity, and 96.8% accuracy vs. CE (92.8% sensitivity, 93.5% specificity, 93.2% accuracy). False negatives occurred mainly in heterozygotes (Premier Resolution: 5\/73 [6.8%] vs. CE: 14\/73 [19.2%]); heterozygous Hb Paks\u00e9 remained undetectable by both techniques (3\/3, 100%). Clinical validation confirmed excellent performance with 94.9% sensitivity, 99.6% specificity, and 99.36% accuracy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Premier Resolution HPLC provides a reliable and cost\u2010effective method for HbCS detection in \u03b1\u2010thalassemia\u2013risk populations. Its enhanced resolution and characteristic chromatographic patterns support routine thalassemia screening in high\u2010prevalence regions, though molecular confirmation remains necessary in heterozygous cases for genetic counseling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70267","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T09:57:14Z","timestamp":1779703034000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Performance Evaluation of a Premier Resolution\n                    <scp>HPLC<\/scp>\n                    System for Detecting Hemoglobin Constant Spring, Hemoglobin Paks\u00e9, and Coexisting \u03b1\u2010 and \u03b2\u2010Thalassemia Mutations"],"prefix":"10.1002","volume":"40","author":[{"given":"Surada","family":"Satthakarn","sequence":"first","affiliation":[{"name":"Department of Medical Technology, Faculty of Allied Health Sciences Burapha University  Chonburi Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wibhasiri","family":"Srisuwan","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences University of Phayao  Phayao Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7589-3849","authenticated-orcid":false,"given":"Sitthichai","family":"Panyasai","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences University of Phayao  Phayao Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/234337a0"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269.2010.526914"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e19116"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269.2011.637149"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2013.12.002"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2009.1.26"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269709000664"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mph.0000243662.56432.37"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16809"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1982.tb02796.x"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05865.x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630261003680191"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2011.01371.x"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPH.0000000000001406"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269.2012.709896"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269.2015.1027828"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2023-1458"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2003.114"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.5001\/omj.2023.78"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.5001\/omj.2024.96"},{"issue":"8","key":"e_1_2_12_22_1","article-title":"Detection of Hemoglobin Constant Spring: A Comparison of Capillary Electrophoresis Versus High\u2010Performance Liquid Chromatography","volume":"16","author":"Embong S. 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