{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:53:46Z","timestamp":1786632826610,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K10573"],"award-info":[{"award-number":["24K10573"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In Japan, clinical laboratories commonly assess lupus anticoagulant (LA) activity using phospholipid\u2010dependent coagulation assays as part of the diagnostic evaluation of antiphospholipid syndrome (APS). However, LA detection involves complex procedures and may yield false\u2010positive results in patients receiving anticoagulant therapy, such as direct oral anticoagulants (DOACs). This study aimed to clarify the effects of anticoagulants on guideline\u2010based LA testing (diluted Russell's viper venom time (dRVVT) and\/or LA\u2010sensitive activated partial thromboplastin time (APTT)) and to evaluate whether they interfere with accurate LA assessment. We also explore a simple one\u2010step method to minimize anticoagulant interference and improve diagnostic efficiency.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We developed a one\u2010step method for assessing LA activity by calculating the LA\u2010sensitivity ratio (LA\u2010SR), defined as the ratio of APTT measured using high\u2010 and low\u2010sensitivity APTT reagents. The clinical utility of the LA\u2010SR assay was evaluated using plasma samples from 57 patients with systemic lupus erythematosus, 74 patients with prolonged APTT, and DOAC\u2010spiked plasmas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The method showed agreement with the guideline\u2010based LA testing. Of the 57 cases, 14 were LA\u2010positive and 38 were LA\u2010negative according to guideline\u2010based testing. Among the 43 cases classified as LA\u2010negative, five showed elevated LA\u2010SR values despite negative guideline\u2010based results. The LA\u2010SR assay was minimally affected by DOACs, warfarin, heparin, or coagulation factor deficiencies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This LA\u2010SR\u2010based method may serve as a useful screening tool for the evaluation of LA activity. A negative LA\u2010SR result may help identify samples unlikely to have LA activity, while positive results should be interpreted cautiously and confirmed using guideline\u2010based testing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70292","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:05:57Z","timestamp":1781759157000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary Evaluation of a Novel Lupus Anticoagulant Activity Assay Unaffected by Anticoagulants"],"prefix":"10.1002","volume":"40","author":[{"given":"Ayu","family":"Hidaka","sequence":"first","affiliation":[{"name":"Department of Laboratory Science, Faculty of Health Science Yamaguchi University Graduate School of Medicine  Ube Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7255-4705","authenticated-orcid":false,"given":"Risa","family":"Kaneshige","sequence":"additional","affiliation":[{"name":"Department of Laboratory Science, Faculty of Health Science Yamaguchi University Graduate School of Medicine  Ube Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seika","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Laboratory Science, Faculty of Health Science Yamaguchi University Graduate School of Medicine  Ube Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukari","family":"Motoki","sequence":"additional","affiliation":[{"name":"Department of Laboratory Science, Faculty of Health Science Yamaguchi University Graduate School of Medicine  Ube 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