{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:20:40Z","timestamp":1788171640389,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YFC2708200"],"award-info":[{"award-number":["2025YFC2708200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2026JJ81843"],"award-info":[{"award-number":["2026JJ81843"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To assess the feasibility of using the cell\u2010free DNA barcode\u2010enabled single\u2010molecule test (cfBEST) for non\u2010invasive prenatal testing (NIPT) of \u03b1\u2010thalassemia and \u03b2\u2010thalassemia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Seventy two thalassemia carrier families participated in the study. The cfBEST method was employed to identify thalassemia genotypes in fetal cell\u2010free DNA extracted from maternal plasma, targeting four \u03b1\u2010thalassemia gene mutations and 13 common \u03b2\u2010thalassemia gene mutations. Validation was conducted using gap\u2010PCR and PCR\u2010RDB through invasive prenatal diagnosis. All prenatal diagnosis results were later confirmed for accuracy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>cfBEST successfully identified 94.6% (88\/93) of fetal alleles, achieving a sensitivity of 94% (95% CI, 83.45%\u201398.75%) and a specificity of 95.35% (95% CI, 84.19%\u201399.43%). All prenatal diagnosis results were followed up, and the follow\u2010up findings were consistent with the prenatal diagnosis results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The findings indicate that cfBEST is a reliable, precise, simple, and cost\u2010efficient method suitable for non\u2010invasive prenatal testing of \u03b1\u2010thalassemia and \u03b2\u2010thalassemia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70298","type":"journal-article","created":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T08:31:18Z","timestamp":1783240278000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of Cell\u2010Free\n                    <scp>DNA<\/scp>\n                    Barcode\u2010Enabled Single\u2010Molecule Test for Non\u2010Invasive Prenatal Testing of \u03b1\u2010Thalassemia and \u03b2\u2010Thalassemia"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8568-3640","authenticated-orcid":false,"given":"Qin","family":"Liu","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Birth Defect for Research and Prevention (Hunan Provincial Maternal and Child Health Care Hospital)  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0089-7789","authenticated-orcid":false,"given":"Na","family":"Ma","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Birth Defect for Research and Prevention (Hunan Provincial Maternal and Child Health Care Hospital)  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanglan","family":"Tang","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Birth Defect for Research and Prevention (Hunan Provincial Maternal and Child Health Care Hospital)  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianting","family":"Chen","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Birth Defect for Research and Prevention (Hunan Provincial Maternal and Child Health Care Hospital)  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deguo","family":"Tang","sequence":"additional","affiliation":[{"name":"Yongzhou Hospital for Maternal and Child Health Care  Yongzhou Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Zeng","sequence":"additional","affiliation":[{"name":"Yongzhou Hospital for Maternal and Child Health Care  Yongzhou Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Wang","sequence":"additional","affiliation":[{"name":"GeneMind Biosciences Co. 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