{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:20:28Z","timestamp":1788171628029,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objectives<\/jats:title>\n To compare the analytical and clinical performance of MAGLUMI X3 (Snibe) and Cobas e602 (Roche) assays for sFlt\u20101, PlGF, and the sFlt\u20101\/PlGF ratio in women hospitalized at the Department of Gynecology and Obstetrics, University Hospital in Pilsen, and to evaluate diagnostic accuracy for early\u2010 and late\u2010onset preeclampsia (PE).<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n This single\u2010center real\u2010world study analyzed 192 serum samples from 164 patients (mean age 31.8\u2009\u00b1\u20095.0\u2009years). Routine testing was performed on Cobas e602; residual serum was measured on MAGLUMI X3 within 24\u2009h. Precision followed CLSI EP05\u2010A3\/EP15\u2010A3; agreement used Passing\u2013Bablok and Bland\u2013Altman. Diagnostic performance was evaluated by ROC curves with AUC and 95% confidence intervals (CI); AUCs were compared by the DeLong test.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Repeatability and reproducibility were acceptable across levels (patient\u2010sample CVs ~0.7%\u20132.0% and 1.1%\u20131.5%; QC CVs ~2.0%\u20138.9%). Bland\u2013Altman showed a mean bias for sFlt\u20101 of \u2212869.9\u2009ng\/L (95% CI \u22121035.6 to \u2212704.2) and for PlGF +6.2\u2009ng\/L (\u22124.6 to 17.0). Spearman's r was 0.99 (sFlt\u20101) and 0.97 (PlGF),\n p<\/jats:italic>\n \u2009<\u20090.0001. For clinical classification, AUCs were high for both platforms\u2014late\u2010onset PE: Roche 0.95 (95% CI 0.86\u20130.99) and Snibe 0.95 (0.87\u20130.99); early\u2010onset PE: Roche 0.85 (0.75\u20130.92) and Snibe 0.84 (0.75\u20130.91)\u2014with no significant differences by DeLong (\n p<\/jats:italic>\n \u2009=\u20090.71 late;\n p<\/jats:italic>\n \u2009=\u20090.32 early).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n MAGLUMI X3 showed acceptable analytical performance and diagnostic discrimination comparable to Cobas e602. Although sFlt\u20101 values were systematically higher on MAGLUMI, overall discrimination remained similar across platforms when platform\u2010specific cut\u2010offs were applied.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcla.70302","type":"journal-article","created":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T08:34:58Z","timestamp":1783240498000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["sFlt<\/scp>\n \u20101\/\n PlGF<\/scp>\n Ratio in the Diagnosis of Preeclampsia on the\n MAGLUMI X3<\/scp>\n Analyzer"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5797-0855","authenticated-orcid":false,"given":"Pavel","family":"Broz","sequence":"first","affiliation":[{"name":"Institute of Clinical Biochemistry and Hematology University Hospital in Pilsen Pilsen Czech Republic"},{"name":"Faculty of Medicine in Pilsen Charles University in Prague Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2338-8090","authenticated-orcid":false,"given":"Simona","family":"Kukralova","sequence":"additional","affiliation":[{"name":"Institute of Clinical Biochemistry and Hematology University Hospital in Pilsen Pilsen Czech Republic"},{"name":"Faculty of Medicine in Pilsen Charles University in Prague Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zdenek","family":"Sebesta","sequence":"additional","affiliation":[{"name":"Institute of Clinical Biochemistry and Hematology University Hospital in Pilsen Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Brslicova","sequence":"additional","affiliation":[{"name":"Institute of Clinical Biochemistry and Hematology University Hospital in Pilsen Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Kozerovsky","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University Hospital in Pilsen Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2868-0269","authenticated-orcid":false,"given":"Jaroslav","family":"Racek","sequence":"additional","affiliation":[{"name":"Institute of Clinical Biochemistry and Hematology University Hospital in Pilsen Pilsen Czech Republic"},{"name":"Faculty of Medicine in Pilsen Charles University in Prague Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0503-7780","authenticated-orcid":false,"given":"Daniel","family":"Rajdl","sequence":"additional","affiliation":[{"name":"Institute of Clinical Biochemistry and Hematology University Hospital in Pilsen Pilsen Czech Republic"},{"name":"Faculty of Medicine in Pilsen Charles University in Prague Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,5]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.l2381"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xagr.2024.100359"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2021.09.008"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000003891"},{"key":"e_1_2_14_6_1","volume-title":"Hypertension in Pregnancy: Diagnosis and Management","author":"National Institute for Health and Care Excellence (NICE)","year":"2019"},{"key":"e_1_2_14_7_1","unstructured":"Clinical & Laboratory Standards Institute \u201cEP09 Compare Measurement Procedures & Estimate Bias \u201davailable from https:\/\/clsi.org\/standards\/products\/method\u2010evaluation\/documents\/ep09."},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000004149"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomed4020010"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.120.15146"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.26855\/ijcemr.2024.04.004"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0263443"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0156013"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2018.06.017"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12884\u2010020\u20102744\u20102"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13715"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2021.12.008"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.120.021376"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2020.03.002"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm\u20102018\u20101228"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e28080"},{"key":"e_1_2_14_22_1","unstructured":"National Institute for Health and Care Excellence (NICE) \u201cOverview: PLGF\u2010based testing to help diagnose suspected preterm pre\u2010eclampsia. NICE Diagnostics Guidance DG49 \u201davailable from NICE Guidance DG49."},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.113.01787"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1414838"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70302","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.70302","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70302","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:08:16Z","timestamp":1788170896000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.70302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,5]]},"references-count":23,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jcla.70302"],"URL":"https:\/\/doi.org\/10.1002\/jcla.70302","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,5]]},"assertion":[{"value":"2025-12-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70302"}}