{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:03:24Z","timestamp":1789653804908,"version":"4.0.0"},"reference-count":43,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102022R1C1C1002741"],"award-info":[{"award-number":["NRF\u20102022R1C1C1002741"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Candida auris<\/jats:italic>\n                      has rapidly spread worldwide, representing a significant global health threat. Rapid diagnostic testing is essential for\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      infection control. This study aimed to assess the diagnostic performance of a direct polymerase chain reaction (PCR) assay compared with culture. The liquid Amies transport medium (Copan ESwab; Copan, Italy) and fungal enrichment broth were compared to determine the optimal media for the direct PCR assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The diagnostic performance of the cobas omni Utility Channel Reagent Kit (Roche Diagnostics, Germany)\u2010based direct PCR assay and culture was assessed against clinical outcomes. Sixty skin swab specimens were prospectively collected from intensive care unit patients at a university hospital in Korea. Each sample was tested in parallel using the Copan ESwab and Sabouraud dextrose (SD) broth.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The direct PCR assay showed a sensitivity of 92.0%\u201396.0% and specificity of 85.7%\u201397.1% compared with a sensitivity of 76.0%\u201380.0% and specificity of 100% for culture across both media. For the direct PCR assay, the Copan ESwab showed a higher specificity, positive predictive value, and kappa value but lower sensitivity and negative predictive value than the SD broth. The discordant direct PCR assay\u2010positive\/reference standard\u2010negative and PCR assay\u2010positive\/culture\u2010negative cases showed significantly higher cycle threshold values than the concordant cases (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The direct PCR assay using the Copan ESwab showed high sensitivity for the detection of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      in clinical skin swab specimens. This approach may be useful for screening, although further validation in larger multicenter studies is needed.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70309","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:09:08Z","timestamp":1783606148000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic Performance of Direct\n                    <scp>PCR<\/scp>\n                    Assay for\n                    <i>Candida auris<\/i>\n                    Detection: A Comparison of Liquid Amies Transport Medium and Fungal Enrichment Broth"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1295-4728","authenticated-orcid":false,"given":"Jung\u2010ah","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Soonchunhyang University Seoul Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Kyung","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8750-4257","authenticated-orcid":false,"given":"Eun Jeong","family":"Won","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"},{"name":"Department of Molecular Genetics and Microbiology Duke University Medical Center  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heungsup","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi\u2010Na","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2008.00083.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00319-11"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/597108"},{"key":"e_1_2_9_5_1","unstructured":"Centers for Disease Control and Prevention (CDC) \u201cTrackingC. auris \u201d(2024) https:\/\/www.cdc.gov\/candida\u2010auris\/tracking\u2010c\u2010auris\/index.html."},{"key":"e_1_2_9_6_1","unstructured":"European Centre for Disease Prevention and Control (ECDC) \u201cSurvey on the Epidemiological Situation Laboratory Capacity and Preparedness for Candidozyma (Candida) Auris 2024 \u201d(2025) https:\/\/www.ecdc.europa.eu\/en\/publications\u2010data\/survey\u2010epidemiological\u2010situation\u2010laboratory\u2010capacity\u2010and\u2010preparedness\u2010candidozyma."},{"key":"e_1_2_9_7_1","unstructured":"World Health Organization (WHO) \u201cGlobal Antimicrobial Resistance Surveillance System (GLASS); 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