{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:00:03Z","timestamp":1790686803621,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000209","name":"National Academy of Sciences","doi-asserted-by":"publisher","award":["DE\u2010HS0000031"],"award-info":[{"award-number":["DE\u2010HS0000031"]}],"id":[{"id":"10.13039\/100000209","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["HHSN261201400009C"],"award-info":[{"award-number":["HHSN261201400009C"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In Hiroshima, thyroid cancer tissue samples from A\u2010bomb survivors have been preserved since 1958. However, the feasibility of using these old formalin\u2010fixed paraffin\u2010embedded (FFPE) specimens for genetic analysis has not been fully evaluated. Here, we aimed to assess the quality of DNA extracted from long\u2010term stored FFPE specimens obtained from patients with papillary thyroid carcinoma (PTC) in Hiroshima and to evaluate their applicability to whole exome sequencing (WES).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We extracted DNA from tumor and adjacent normal regions of FFPE thyroid tumor specimens from 57 cases stored for up to 55\u2009years at three hospitals in Hiroshima and confirmed the quality of the extracted DNA. From this cohort, tumor and paired normal samples from 24 patients with PTC were selected for WES, and sequencing metrics were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Both DNA yield and DNA integrity number were negatively correlated with storage duration. WES analysis showed that storage duration was also negatively correlated with mean insert size and normalized read depth. In contrast, the uniformity score, which reflects the evenness of sequence coverage across targeted regions, did not show a significant correlation with storage duration. Among the 22\n                      <jats:italic>BRAF<\/jats:italic>\n                      V600E\u2010positive cases identified by droplet digital PCR, 18 cases (82%) had at least one sequencing read supporting the mutation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings suggest that FFPE samples preserved for up to 55\u2009years may be amenable to sequencing with increased read depth. However, the progressive decline in DNA quality with longer storage periods indicates that careful interpretation of sequencing results is warranted.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70324","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T03:49:29Z","timestamp":1785469769000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["DNA Extracted From Aged Formalin\u2010Fixed Paraffin\u2010Embedded Specimens of Patients With Papillary Thyroid Carcinomas Can Be Used for Whole Exome Sequencing"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0826-2940","authenticated-orcid":false,"given":"Kousuke","family":"Tanimoto","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences Radiation Effects Research Foundation  Hiroshima Hiroshima Japan"},{"name":"Biosample Research Center Radiation Effects Research Foundation  Hiroshima Hiroshima Japan"},{"name":"Department of High\u2010risk Infectious Disease Control, Graduate School of Medical and Dental Sciences Institute of Science Tokyo  Bunkyo\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7929-5355","authenticated-orcid":false,"given":"Tomonori","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences Radiation Effects Research Foundation  Hiroshima Hiroshima Japan"},{"name":"Biosample Research Center Radiation Effects Research Foundation  Hiroshima Hiroshima Japan"},{"name":"Research Center for Prevention From Radiation Hazards of Workers National Institute of Occupational Safety and Health  Kawasaki Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidewaki","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Laboratory for Cancer Genomics RIKEN Center for Integrative Medical 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