{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:58:56Z","timestamp":1790686736501,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102025\u201000519514"],"award-info":[{"award-number":["RS\u20102025\u201000519514"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Minimal residual disease (MRD) assessment by next\u2010generation flow cytometry (NGF) is an important prognostic tool in multiple myeloma (MM). Although the EuroFlow NGF assay provides highly standardized and sensitive MRD detection, its dual\u2010tube workflow requires larger sample volumes and longer processing time, potentially limiting efficiency in routine clinical practice. This prospective study evaluated a 10\u2010color single\u2010tube (ST\u201010C) MRD assay by directly comparing its analytical and clinical performance with the EuroFlow NGF assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>MRD analysis was performed in 40 patients with MM, of whom 39 who achieved very good partial response or complete response were included in the final analysis. Each sample was analyzed in parallel using both assays. Analytical sensitivity, qualitative and quantitative agreement, and progression\u2010free survival (PFS) were assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Both assays achieved analytical sensitivity below 10\n                      <jats:sup>\u22125<\/jats:sup>\n                      . MRD was detected in 48.7% of samples by the ST\u201010C assay and in 38.5% by the EuroFlow NGF assay, with substantial agreement (\u03ba\u2009=\u20090.79). Concordance remained high using the clinical MRD cutoff (\u2265\u20090.001%) (\u03ba\u2009=\u20090.84). Quantitative MRD levels showed strong correlation between the assays (\u03c1\u2009=\u20090.964) without significant systematic bias. MRD\u2010positive patients showed a trend toward inferior PFS compared with MRD\u2010negative patients by both assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The ST\u201010C assay demonstrated analytical performance comparable to the EuroFlow NGF assay, supporting its adoption as a more efficient and streamlined alternative for routine MRD assessment in MM.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70332","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T08:16:58Z","timestamp":1786436218000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prospective Head\u2010To\u2010Head Comparison of a 10\u2010Color Single\u2010Tube Flow Cytometry and\n                    <scp>EuroFlow<\/scp>\n                    Next\u2010Generation Flow in Multiple Myeloma Minimal Residual Disease"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5966-5566","authenticated-orcid":false,"given":"Go Eun","family":"Bae","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Genetics Samsung Medical Center, Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae Jin","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics Samsung Medical Center, Sungkyunkwan University School of Medicine  Seoul Republic of Korea"},{"name":"Department of Health and Safety Convergence Science Korea University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Hoan","family":"Bang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics Samsung Medical Center, Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhng\u2010Wook","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Health and Safety Convergence Science Korea University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3408-767X","authenticated-orcid":false,"given":"Ari","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics Samsung Medical Center, Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee\u2010Jin","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics Samsung Medical Center, Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1499-7131","authenticated-orcid":false,"given":"Jun Ho","family":"Yi","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Medicine, Samsung Medical Center Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kihyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Medicine, Samsung Medical Center Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6861-3282","authenticated-orcid":false,"given":"Duck","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics Samsung Medical Center, Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0553-7096","authenticated-orcid":false,"given":"Hyun\u2010Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics Samsung Medical Center, Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-11-568907"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021011101"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2023.0298"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(16)30206-6"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2022.42.5.558"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.29"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2016003715"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-021-04634-5"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqw052"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2019.238394"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2026.41.e5"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21265"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.708231"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-89761-9"}],"container-title":["Journal 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