{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:50:33Z","timestamp":1791989433054,"version":"4.4.0"},"reference-count":52,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1K07CA225403"],"award-info":[{"award-number":["1K07CA225403"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21DE031516"],"award-info":[{"award-number":["R21DE031516"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006671","name":"Maryland Department of Health and Mental Hygiene","doi-asserted-by":"publisher","award":["CH\u2010649\u2010CRF"],"award-info":[{"award-number":["CH\u2010649\u2010CRF"]}],"id":[{"id":"10.13039\/100006671","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH\u201011\u20102\u20100174"],"award-info":[{"award-number":["W81XWH\u201011\u20102\u20100174"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH\u201018\u20102\u20100040"],"award-info":[{"award-number":["W81XWH\u201018\u20102\u20100040"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["HT9425\u201024\u20103\u20100004"],"award-info":[{"award-number":["HT9425\u201024\u20103\u20100004"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Human papillomavirus (HPV)\u2010associated cancers are preventable with vaccination. Serologic assays monitor antibody responses, but their complexity and cost pose limitations when evaluating population level immunity. We developed an L1\u2010based assay for the detection of multiple type\u2010specific HPV antibodies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The Luminex\u2010based assay used recombinant L1 proteins from seven HPVs (HPV\u20106\/11\/16\/18\/35\/45\/58) for the detection of type\u2010specific immunoglobulin (Ig)G and IgA. Cutoffs were established using plasma from infants and children. The assay was optimized and qualified using a sample set from a blinded proficiency panel developed at the Frederick National Laboratory for Cancer Research.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Sensitivity and specificity for anti\u2010HPV\u20106\/11\/16\/18\/58 IgG were 100% at the Mean\u2009+\u20093 standard deviation [SD] and Mean\u2009+\u20095\u2009SD thresholds. Sensitivity for HPV\u201045 was 100% and 92.9% at the +3\u2009SD and +5\u2009SD thresholds, respectively. IgA detection showed moderate positivity among vaccinated individuals, with peak estimates of 96.4% (HPV\u201011) and 85.7% (HPV\u201058) at the +3\u2009SD threshold. IgA was correlated with IgG (Spearman's\n                      <jats:italic>\u03c1<\/jats:italic>\n                      \u2009=\u20090.74). The L1\u2010assay differentiated high, intermediate, low, and negative response groups from the blinded samples for all HPV genotypes (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The L1\u2010binding assay has the potential to be a scalable option for high\u2010throughput detection of broad\u2010spectrum HPV immunoglobulins, but further standardization and validation are needed.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70343","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:39:47Z","timestamp":1788327587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary Development and Assessment of a Multiplex Assay for Human Papillomavirus Antibody Detection Using Commercial\n                    <scp>L1<\/scp>\n                    Proteins"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4229-5153","authenticated-orcid":false,"given":"Arash","family":"Aslanabadi","sequence":"first","affiliation":[{"name":"Institute of Human Virology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Karimi","sequence":"additional","affiliation":[{"name":"Institute of Human Virology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Powell","sequence":"additional","affiliation":[{"name":"Institute of Human Virology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8454-9660","authenticated-orcid":false,"given":"Lisa","family":"Schumaker","sequence":"additional","affiliation":[{"name":"University of Maryland Greenebaum Comprehensive Cancer Center University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley","family":"Shutt","sequence":"additional","affiliation":[{"name":"Institute of Human Virology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5381-9261","authenticated-orcid":false,"given":"Mahsa","family":"Hojabri","sequence":"additional","affiliation":[{"name":"Institute of Human Virology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8976-9634","authenticated-orcid":false,"given":"Rahim","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Institute of Human Virology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren M.","family":"Bentzen","sequence":"additional","affiliation":[{"name":"University of Maryland Greenebaum Comprehensive Cancer Center University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin J.","family":"Cullen","sequence":"additional","affiliation":[{"name":"University of Maryland Greenebaum Comprehensive Cancer Center University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5947-265X","authenticated-orcid":false,"given":"Trevor A.","family":"Crowell","sequence":"additional","affiliation":[{"name":"U.S. Military HIV Research Program, CIDR Walter Reed Army Institute of Research  Silver Spring Maryland USA"},{"name":"Henry M. 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