{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T11:41:24Z","timestamp":1792755684230,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20231184"],"award-info":[{"award-number":["BK20231184"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013254","name":"National College Students Innovation and Entrepreneurship Training Program","doi-asserted-by":"publisher","award":["202210313033Z"],"award-info":[{"award-number":["202210313033Z"]}],"id":[{"id":"10.13039\/501100013254","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013254","name":"National College Students Innovation and Entrepreneurship Training Program","doi-asserted-by":"publisher","award":["202410313028Z"],"award-info":[{"award-number":["202410313028Z"]}],"id":[{"id":"10.13039\/501100013254","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n (KP) is an important and virulent pathogen causing acute respiratory infections in infants. Currently, traditional methods for detecting\n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n in babies are labour\u2010intensive and time\u2010consuming, and their accuracy and sensitivity require improvement. Recombinant enzyme polymerase amplification technology (RPA) has the advantages of good specificity, high sensitivity, and isothermal amplification. It can be combined with fluorescence immunochromatography test strip (FICTS) based on nanofluorescent microspheres (nFM) to achieve rapid, low\u2010cost, high sensitivity, and specific detection of\n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n in infants.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n mdh<\/jats:italic>\n was selected as a genus\u2010specific gene from\n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n , and recombinant polymerase isothermal amplification\u2014nanofluorescence microsphere immunochromatography (RPA\u2010FICTS) was established by using standard strains. The sensitivity and specificity of this detection technology can be improved by the magnetic bead purification step of RPA nucleic acid amplification products. Subsequently, rapid quantitative detection of\n \n K. pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n in infant samples was performed using fluorescent immunochromatographic test strips. A total of 19 clinical samples of\n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n (KP) and 7 clinical samples of non\u2010\n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n (nKP) were detected by the new detection technology platform established in this paper, and the specificity and sensitivity of RPA\u2010FICTS were verified by agarose gel electrophoresis.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Under the optimal detection conditions, the maximum detection limits of RPA\u2010GE (agarose gel electrophoresis) and RPA\u2010FICTS are 9.03\u2009\u00d7\u200910\n \u22123<\/jats:sup>\n and 9.03\u2009\u00d7\u200910\n \u22128<\/jats:sup>\n \u2009ng\/\u03bcL. RPA\u2010FICTS can quickly detect\n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n and specifically distinguish between\n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n and non\u2010\n \n Klebsiella\n pneumoniae<\/jats:styled-content>\n <\/jats:italic>\n . Compared with traditional detection methods, this method has a shorter detection time (55\u2009min), strong specificity, high sensitivity, and simple operation, providing a new method for rapid detection of human\n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcla.70348","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T06:36:49Z","timestamp":1791527809000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Detection of\n Klebsiella pneumoniae<\/i>\n in Infants With Severe Infection Disease by Instant Nanofluorescent Microspheres\u2010Immunochromatography Combined With Recombinase Polymerase Isothermal Amplification Technology"],"prefix":"10.1002","volume":"40","author":[{"given":"Yiping","family":"Liu","sequence":"first","affiliation":[{"name":"School of Medical Technology Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7742-7818","authenticated-orcid":false,"given":"Yi","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Medical Technology Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering and Technology China University of Mining and Technology Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8487-0799","authenticated-orcid":false,"given":"Hanzhong","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xuzhou First People's Hospital Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumeng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xuzhou First People's Hospital Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5985-6186","authenticated-orcid":false,"given":"Minghui","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medical Imaging Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifan","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Medical Technology Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6985-692X","authenticated-orcid":false,"given":"Xing","family":"Lv","sequence":"additional","affiliation":[{"name":"School of Medical Technology Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Xuzhou First People's Hospital Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4109-2808","authenticated-orcid":false,"given":"Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medical Technology Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"569","article-title":"Investigation and Control on a Suspected Outbreak of Carbapenem\u2014Resistant \n Klebsiella pneumoniae\n Infection in the Neurological Intensive Care Unit","volume":"22","author":"Ying H.","year":"2023","journal-title":"Chinese Journal of Infection Control"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1144463"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.634349"},{"key":"e_1_2_11_5_1","first-page":"362","article-title":"Clinical Characteristics,Molecular Typing and Drug Resistance Genes of Carbapenem\u2010Resistant \n Klebsiella pneumoniae\n Isolated From Blood Culturing","volume":"38","author":"Jiawei W.","year":"2023","journal-title":"Laboratory Medicine"},{"key":"e_1_2_11_6_1","first-page":"22","article-title":"Recent Progress on Isothermal Amplification Technology and Its Combination With CRISPR in Rapid Detection of Microorganisms","volume":"36","author":"Wei\u2010fang G.","year":"2020","journal-title":"Biotechnology Bulletin"},{"key":"e_1_2_11_7_1","first-page":"4479","article-title":"Application of CRISPR\u2010Cas12a in the Detection of Foodborne Pathogens","volume":"13","author":"Bu X. 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