{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T11:41:26Z","timestamp":1792755686343,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Acquired factor V (FV) inhibitors are rare circulating autoantibodies that may cause severe bleeding and false\u2010negative detection without standardized incubation. This study aimed to evaluate time\u2010 and temperature\u2010dependent FV inhibitor activity and define optimal laboratory detection conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fifteen serially diluted plasma samples from 4 FV inhibitor\u2010positive patients were incubated at room temperature (RT) or 37\u00b0C for 0\u2013120\u2009min. APTT mixing assays, residual FV activity, PT mixing assays, and Bethesda inhibitor titers were detected, with inhibitor\u2010free normal pooled plasma (NPP) as thermal degradation control. ISTH\u2010recommended MTC index was adopted for standardized mixing result interpretation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      At 37\u00b0C, APTT\/PT mixing times were progressively prolonged and residual FV activity declined continuously, reaching a plateau at 60 min, whereas only slight changes occurred at RT. After excluding ~6% spontaneous FV thermal loss in NPP, residual FV activity at 37\u00b0C was significantly lower than RT at all time points (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Bethesda titers rose progressively at 37\u00b0C across all samples, confirming antibody\u2010mediated neutralization rather than native protein decay. Pronounced inter\u2011individual variability in inhibitor potency was observed under equalized inhibitor concentrations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>FV inhibitors exhibit distinct time\u2010 and temperature\u2010dependent neutralizing activity. RT testing without sufficient 37\u00b0C incubation raises false\u2010negative risk. Universal 2\u2009h incubation at 37\u00b0C is recommended for routine inhibitor screening; 60\u2009min incubation is only suitable for follow\u2010up of confirmed FV inhibitor cases and cannot replace standard 2\u2010h protocol for ruling out FVIII inhibitors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70353","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:26:48Z","timestamp":1789478808000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Time\u2010 and Temperature\u2010Dependent Response of Acquired\n                    <scp>FV<\/scp>\n                    Inhibitors: Implications for Laboratory Diagnosis and Clinical Management"],"prefix":"10.1002","volume":"40","author":[{"given":"Lihua","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Fujian Medical University Union Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6211-3575","authenticated-orcid":false,"given":"Mingjie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Fujian Medical University Union Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Fujian Medical University Union Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junxiang","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Engineering Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingping","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Fujian Medical University Union Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9978-9509","authenticated-orcid":false,"given":"Lihui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Fujian Pingtan Comprehensive Experimental Area Hospital  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Fujian Medical University Union Hospital Fujian Medical University  Fuzhou 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