{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:52:17Z","timestamp":1791276737688,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Lipemia is a common pre\u2010analytical interference in clinical chemistry. Triglyceride (TG) concentration is commonly used as a surrogate indicator for lipemic interference, but its predictive reliability is limited by heterogeneity in lipoprotein particle composition. This study aimed to establish and validate a quantitative Lipemia index (LI) assay for direct measurement of serum turbidity, compare its predictive value with TG, and evaluate its clinical applicability through reference interval estimation and correlation with interference deviations in native patient samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The LI assay was developed on a Beckman Coulter AU5800 analyzer by measuring \u0394\n                      <jats:italic>A<\/jats:italic>\n                      (\n                      <jats:italic>A<\/jats:italic>\n                      <jats:sub>660<\/jats:sub>\n                      \u2013\n                      <jats:italic>A<\/jats:italic>\n                      <jats:sub>800 nm<\/jats:sub>\n                      ). Performance was validated per CLSI guidelines: precision (EP05\u2010A3), detection capability (EP17\u2010A2), linearity (EP06\u2010ED2), method comparison (Passing\u2010Bablok regression, Bland\u2013Altman analysis), reference interval (EP28\u2010A3c), and dilution recovery (EP07\u2010A2). LI's predictive value for lipemic interference was compared to TG using deviations in total protein assays (single\u2010 vs. double\u2010reagent methods).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The LI assay demonstrated precision with CV \u2264\u20092.23% and linearity (0.05\u201330.87\u2009mmol\/L TG\u2010equivalent). It correlated strongly with the reference TG method (\n                      <jats:italic>\u03c1<\/jats:italic>\n                      \u2009=\u20090.998,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). In predicting assay interference, LI showed a closer correlation with total protein deviations than did TG (\n                      <jats:italic>r<\/jats:italic>\n                      <jats:sub>s<\/jats:sub>\n                      \u2009=\u20090.822 vs. 0.770). A reference interval of 0.093\u20130.240 was established for healthy adults. Dilution recovery (90%\u2013110%) confirmed accuracy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The quantitative LI assay provides a standardized, clinically validated measure of lipemic interference aligning more directly with the optical nature of clinical chemistry testing than TG measurement. These findings support its analytical validity, utility as a predictor of turbidity interference, and clinical applicability.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.70378","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:17:48Z","timestamp":1791274668000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment and Application of a Quantitative Lipemia Index as a Novel Indicator for Assessing Lipemic Interference in Clinical Chemistry"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4462-9997","authenticated-orcid":false,"given":"Haifeng","family":"Xie","sequence":"first","affiliation":[{"name":"Laboratory Medicine Center Hangzhou Traditional Chinese Medicine (TCM) Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhua","family":"Bao","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Center Hangzhou Traditional Chinese Medicine (TCM) Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengcheng","family":"Tong","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Center Hangzhou Traditional Chinese Medicine (TCM) Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"Diagnostics Division Beckman Coulter (China) Co., Ltd. (North China Region)  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Tonghua Central Hospital  Tonghua China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3987-5651","authenticated-orcid":false,"given":"Jinlong","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Central Hospital of Dalian University of Technology  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1373\/clinchem.2004.038075","article-title":"Evaluating Interference Caused by Lipemia","volume":"50","author":"Kroll M. 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