{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T05:24:45Z","timestamp":1751865885245},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,10,10]],"date-time":"2001-10-10T00:00:00Z","timestamp":1002672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effects of calcium, calmodulin, protein kinase C (PKC) and protein tyrosine kinase (PTK) modulators were examined on the volume\u2010activated taurine efflux in the erythroleukemia cell line K562. Exposure to hypoosmotic solution significantly increased taurine efflux and intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>). The Ca<jats:sup>2+<\/jats:sup> channel blockers La<jats:sup>3+<\/jats:sup> (1 mM), verapamil (200 \u03bcM) and nifedipine (100 \u03bcM) inhibited the hypoosmotically\u2010induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase by more than 90%, while the volume\u2010activated taurine efflux was inhibited by 61.3\u2009\u00b1\u20099.5, 74.1\u2009\u00b1\u20099.3 and 38.0\u2009\u00b1\u20091.5%, respectively. Furthermore, the calmodulin inhibitors W7 (50 \u03bcM) and trifluoperazine (10 \u03bcM) and the Ca<jats:sup>2+<\/jats:sup>\/calmodulin\u2010dependent protein kinase II inhibitor KN\u201062 (2 \u03bcM) significantly blocked the volume\u2010activated taurine efflux by 93.4\u2009\u00b1\u20092.7, 77.9\u2009\u00b1\u20093.5 and 61.3\u2009\u00b1\u200915.8%, respectively. In contrast, the PKC inhibitor staurosporine (200 nM) or the PKC activator phorbol 12\u2010myristate 13\u2010acetate (100 nM) did not have significant effects on the volume\u2010activated taurine efflux. However, pretreatment with PTK inhibitors genistein, tyrphostin A25, and tyrphostin A47 blocked the volume\u2010activated taurine efflux. These results suggest that the volume\u2010activated taurine efflux in K562 cells may not directly involve Ca<jats:sup>2+<\/jats:sup>, but may require the presence of calmodulin and\/or PTK. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.10027","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T19:52:11Z","timestamp":1030305131000},"page":"316-322","source":"Crossref","is-referenced-by-count":9,"title":["Effects of calcium, calmodulin, protein kinase C and protein tyrosine kinases on volume\u2010activated taurine efflux in human erythroleukemia cells"],"prefix":"10.1002","volume":"189","author":[{"given":"Chiun\u2010Chien","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chirn\u2010Bin","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jer\u2010Yuh","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srisaila","family":"Basavappa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poh\u2010Hong","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02740638"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1152\/jn.1996.76.2.764","article-title":"Swelling\u2010activated amino acid efflux in the human neuroblastoma cell line CHP\u2010100","volume":"76","author":"Basavappa S","year":"1996","journal-title":"J Neurophysiol"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1992.tb10064.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021928"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.5.C1457"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-2-00291.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.2.C515"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869333"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.3.C728"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.1.C57"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402540116"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1989.69.2.315"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199605)167:2<354::AID-JCP21>3.0.CO;2-6"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240000260"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(89)90019-1"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.2.C671"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240000296"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)81630-I"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"29389","DOI":"10.1016\/S0021-9258(18)43891-4","article-title":"Inhibition of volume\u2010activated I\u2010 and taurine efflux from HeLa cells by P\u2010glycoprotein blockers correlates with calmodulin inhibition","volume":"269","author":"Kirk J","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"23475","DOI":"10.1016\/S0021-9258(18)35862-9","article-title":"Transport of organic substances via a volume\u2010activated channel","volume":"267","author":"Kirk K","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V56.3.344.344"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233436"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.1.247"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402420113"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","first-page":"5026","DOI":"10.1002\/j.1460-2075.1994.tb06831.x","article-title":"Visualization of neural control of intracellular Ca2+ concentration in single vascular smooth muscle cells in situ","volume":"13","author":"Lino M","year":"1994","journal-title":"EMBO J"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V45.3.321.321"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199511093331906"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.4.C823"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.5.C1226"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19970115)47:2<144::AID-JNR3>3.0.CO;2-F"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1998.274.6.R1677"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-06-02638.1993"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.3.C755"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01373008"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440030514"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233740"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01474677"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1998.tb03346.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00410189"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(05)80337-5"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)80672-3"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.4.C1056"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.341bw.x"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.10027","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.10027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T06:08:26Z","timestamp":1696918106000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.10027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,10]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1002\/jcp.10027"],"URL":"https:\/\/doi.org\/10.1002\/jcp.10027","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,10]]}}}