{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:10:02Z","timestamp":1718651402054},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,12,18]],"date-time":"2002-12-18T00:00:00Z","timestamp":1040169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The biology of prostanoids in the normal human colon is only beginning to be understood. We used in situ and in vitro techniques to define the lineage, number, per cell enzyme content, and epithelial functional effect of prostaglandin\u2010generating cells, identified by the presence of cyclooxygenase 1 (COX 1). Immunohistochemical results were quantitated densitometrically, and cell surface staining in situ was verified by flow cytometry of isolated cells and by Western blotting. Three populations of COX 1<jats:sup>+<\/jats:sup> mucosal cells were identified, based on their morphology and local distribution in human mucosa; these were in the intra\u2010epithelial, crypt apical, and lamina propria regions, with each containing a similar amount of COX 1 protein on a per cell basis. The most numerous were COX 1<jats:sup>+<\/jats:sup> mononuclear cells in the lamina propria, identified as CD3<jats:sup>+<\/jats:sup> T lymphocytes, both in situ and ex vivo. In toto, 21% of lamina propria mononuclear cells were COX 1<jats:sup>+<\/jats:sup>, and over 50% of these cells were CD3<jats:sup>+<\/jats:sup> T cells. Findings were similar in the colon with mild\u2010moderate inflammation due to ulcerative colitis. Using established surface markers, intra\u2010epithelial and crypt apical COX 1<jats:sup>+<\/jats:sup> cells were non\u2010lymphoid CD45<jats:sup>+<\/jats:sup> leukocytes; neither IgA (B\u2010lymphocytes) nor \u03b1\u2010smooth muscle actin (myelofibroblasts) was co\u2010expressed on these COX 1<jats:sup>+<\/jats:sup> cells. Examining the effect of a major product of COX 1 in an in vitro system of human colonic epithelial monolayers, prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) in low concentration (10<jats:sup>\u22126<\/jats:sup> M) enhanced epithelial barrier function and partially protected epithelia from the barrier\u2010disruptive consequences of a pro\u2010inflammatory cytokine, IFN\u2010\u03b3. We conclude that the human colon contains three tiers of cell types for local synthesis of prostanoids, distinguishable by their location, morphology, and cell lineage. Further, maintenance of the barrier function of colonic epithelium may be added to other cell functions in mucosa regulated, in part, by prostanoids. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.10240","type":"journal-article","created":{"date-parts":[[2002,12,20]],"date-time":"2002-12-20T14:09:29Z","timestamp":1040393369000},"page":"176-185","source":"Crossref","is-referenced-by-count":3,"title":["Peri\u2010epithelial origin of prostanoids in the human colon"],"prefix":"10.1002","volume":"194","author":[{"given":"Kate","family":"McCarn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenna","family":"Yursik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharif","family":"Halim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James K.","family":"Roche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.4049\/jimmunol.150.6.2356","article-title":"Gamma interferon modulation of epithelial barrier function: time course, reversibility and site of cytokine binding","volume":"150","author":"Adams RB","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199601)166:1<130::AID-JCP15>3.0.CO;2-J"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108719"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112311"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90296-1"},{"key":"e_1_2_5_7_1","first-page":"169","volume-title":"Principles of pharmacology","author":"Feuerstein EZ","year":"1996"},{"key":"e_1_2_5_8_1","first-page":"107","volume-title":"Immunology and immunpathology of the liver and the gastrointestinal tract","author":"Fiocchi C","year":"1986"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-198803000-00018"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/S0016-5085(81)80021-2","article-title":"Incidence rates of ulcerative colitis and Crohn's disease in fifteen areas of the United States","volume":"81","author":"Garland CF","year":"1981","journal-title":"Gastroenterology"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90128-8"},{"key":"e_1_2_5_12_1","first-page":"61","article-title":"Effect of the prostaglandin analogue misoprostol on cell proliferation in the canine small intestine","volume":"76","author":"Goodlad RA","year":"1991","journal-title":"Emp Physiol"},{"key":"e_1_2_5_13_1","first-page":"13","article-title":"Leukocyte common antigen expression on T cells in normal and inflamed gut","volume":"68","author":"Harvey J","year":"1989","journal-title":"Immunology"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.4.C1262"},{"key":"e_1_2_5_15_1","first-page":"1735","volume-title":"Gastrointestinal and liver disease","author":"Jewell DP","year":"1997"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-107-4-513"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00271349"},{"key":"e_1_2_5_18_1","first-page":"669","volume-title":"The pharmocological basis of therapeutics","author":"Morrow JD","year":"2001"},{"key":"e_1_2_5_19_1","first-page":"281","volume-title":"Inflammatory bowel diseases","author":"Morson BC","year":"1980"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198307000-00004"},{"key":"e_1_2_5_21_1","doi-asserted-by":"crossref","first-page":"5730","DOI":"10.4049\/jimmunol.153.12.5730","article-title":"Regulation of intestinal epithelial barrier function by TGF\u2010\u03b21","volume":"153","author":"Planchon S","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199910)181:1<55::AID-JCP6>3.0.CO;2-M"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.21.7.591"},{"key":"e_1_2_5_24_1","first-page":"216","volume-title":"Pharmacology","author":"Rand HP","year":"1999"},{"key":"e_1_2_5_25_1","first-page":"614","article-title":"Mucosal synthesis and release of PGE2, from activated eosinophils and macrophages in ulcerative colitis","volume":"90","author":"Raub Y","year":"1995","journal-title":"Am J Gastroenterol"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(92)90142-G"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111728"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(93)90569-2"},{"key":"e_1_2_5_29_1","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/S0016-5085(19)31672-5","article-title":"Role of prostaglandins in ulcerative colitis: Enhanced production during active disease and inhibition by sulfasalazine","volume":"75","author":"Sharon P","year":"1978","journal-title":"Gastro"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70196-9"},{"key":"e_1_2_5_31_1","first-page":"581","volume-title":"Gastrointestinal disease","author":"Soll AH","year":"1993"},{"key":"e_1_2_5_32_1","first-page":"7","article-title":"Expression of T\u2010cell receptors TcR1 (\u03b3\/\u03b4) and TcR2 (\u03b1\/\u03b2) in the human intestinal mucosa","volume":"68","author":"Trejdosiewcz LK","year":"1989","journal-title":"Immunology"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.24.7.659"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.10240","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T16:05:51Z","timestamp":1696867551000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.10240"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12,18]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,2]]}},"alternative-id":["10.1002\/jcp.10240"],"URL":"https:\/\/doi.org\/10.1002\/jcp.10240","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12,18]]}}}