{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:11:57Z","timestamp":1764688317821},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T00:00:00Z","timestamp":1047427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neural stem cells (NSCs) could be very useful for the \u201ccell therapy\u201d treatment of neurological disorders. For this reason basic studies aiming to well characterize the biology of NSCs are of great interest. We carried out a molecular and immunocytochemical analysis of EGF\u2010responsive NSCs obtained from rat pups. After the initial growth of NSCs as floating neurospheres in EGF\u2010containing medium, cells were plated on poly\u2010<jats:sc>L<\/jats:sc>\u2010ornithine\u2010coated dishes either in the presence or absence of EGF. We followed cell differentiation and apoptosis for 21 days in vitro and analyzed the expression levels of some genes having a major role in these processes, such as <jats:italic>pRB<\/jats:italic>, <jats:italic>pRB2<\/jats:italic>\/<jats:italic>p130<\/jats:italic>, <jats:italic>p27<\/jats:italic>, and <jats:italic>p53<\/jats:italic>. We observed that EGF impairs neuronal differentiation. Furthermore, in the absence of mitogens, apoptosis, which appeared to proceed through the \u201cp53 network,\u201d was significantly lower than in the presence of EGF. The cyclin kinase inhibitor p27, while important for cell cycle exit, seemed dispensable for cell survival and differentiation. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.10249","type":"journal-article","created":{"date-parts":[[2003,3,19]],"date-time":"2003-03-19T11:59:32Z","timestamp":1048075172000},"page":"220-233","source":"Crossref","is-referenced-by-count":38,"title":["EGF\u2010responsive rat neural stem cells: Molecular follow\u2010up of neuron and astrocyte differentiation in 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