{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T04:25:14Z","timestamp":1770524714270,"version":"3.49.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,7,31]],"date-time":"2003-07-31T00:00:00Z","timestamp":1059609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Earlier studies demonstrated that knock\u2010out of fibroblast growth factor\u20105 gene <jats:italic>(Fgf\u20105)<\/jats:italic> prolonged anagen VI phase of hair cycle, resulting long hairs in the mice. We showed the activities on hair growth of the two <jats:italic>Fgf\u20105<\/jats:italic> gene products, one of which, FGF\u20105 suppressed hair growth by inhibiting anagen proceeding and inducing the transition from anagen to catagen, and FGF\u20105S, a shorter polypeptide with FGF\u20105\u2010antagonizing activity translated from alternatively spliced mRNA, suppressed this activity of FGF\u20105. As the results suggested that FGF\u20105 antagonist would increase hair growth, we synthesized various peptides having partial sequences of human FGF\u20105 and FGF\u20105S and determined their FGF\u20105 antagonist activity. Among them, a decapeptide designated P3 (95\u2010VGIGFHLQIY\u2010104) that aligns with receptor binding sites of FGF\u20101 and FGF\u20102 suppressed FGF\u20105\u2010induced proliferation of BALB\/3T3 A31 and NIH\/3T3 murine fibroblasts, and FGF receptor\u20101c (FGFR\u20101c)\u2010transfected Ba\/F3 cell line (FR\u2010Ba\/F3 cells). IC50s of this peptide on these cell proliferations were 64, 28, 146 \u03bcM, respectively. On the other hand, IC50 of this peptide on binding of FGF\u20105 to the FGFR\u20101(IIIc)\/Fc chimera was 483 \u03bcM. Examination in dorsal depilated mice revealed that the P3 peptide reduced the activity of FGF\u20105 to recover hair pigmentation and hair follicle lengths. The classification of histologically observed skin sections showed FGF\u20105\u2010induced delations of anagen procedure had reduced by the P3 peptide. The anti\u2010Ki67 antibody staining of hair follicles was inhibited by administration of FGF\u20105, and this inhibition by FGF\u20105 was recovered by administration of the P3 peptide. The P3 peptide alone did not affect hair follicle length and hair cell proliferation. These results indicate that the decapeptide antagonized FGF\u20105 activity in vivo, and reduced the inhibition of FGF\u20105 in hair growth, confirming that FGF\u20105 inhibitors are promising substances against hair loss and\/or for promoting hair growth. J. Cell. Physiol. 197: 272\u2013283, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.10369","type":"journal-article","created":{"date-parts":[[2003,9,16]],"date-time":"2003-09-16T13:35:58Z","timestamp":1063719358000},"page":"272-283","source":"Crossref","is-referenced-by-count":26,"title":["Decapeptide with fibroblast growth factor (FGF)\u20105 partial sequence inhibits hair growth suppressing activity of FGF\u20105"],"prefix":"10.1002","volume":"197","author":[{"given":"Chikako","family":"Ito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuko","family":"Saitoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuko","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimitsu","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Imamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syuichi","family":"Oka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,8,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"348","DOI":"10.2340\/0001555576348352","article-title":"Immunohistochemical studies of proinflammatory cytokines and their receptors in hair follicles of normal human skin","volume":"76","author":"Ahmed AA","year":"1996","journal-title":"Acta Derm Venereol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60305-X"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1071\/BI9880261"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1954.34.1.113"},{"key":"e_1_2_5_6_1","first-page":"1311","article-title":"Activation of fibroblast growth factor (FGF) receptors by recombinant human FGF\u20105","volume":"8","author":"Clements DA","year":"1993","journal-title":"Oncogene"},{"key":"e_1_2_5_7_1","first-page":"145","article-title":"Keratinocyte growth factor is an important endogenous mediator of hair follicle growth, development, and differentiation. 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