{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T21:38:37Z","timestamp":1766266717836},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":11875,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1972,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In extracts of normal and Lesch\u2010Nyhan (LN) heterozygous skin fibroblast monolayer cultures, hypoxanthine\u2010guanine (H\u2010G) and adenine (A) phosphoribosyltransferase (PRT) activities are correlated. These activities vary concomitantly during the life cycle of a culture: Peak activities occur during exponential growth. The ratio of H\u2010GPRT to APRT activity can manifest heterozygosity at the LN locus when H\u2010GPRT activity, per se, is close to and unreliable different from the normal range.<\/jats:p><jats:p>If 2 or 8 \u00d7 10<jats:sup>4<\/jats:sup> cells are planted per 35 mm petri dish, a strain with clearly heterozygous levels of H\u2010GPRT activity in its cell extract may not reveal its genotype by simultaneous measurement of adenine and hypoxanthine incorporation into its acid\u2010insoluble fraction one day after subculture. In contrast, a 1:1 coculture of normal and mutant cells yields the ratio of adenine to hypoxanthine incorporation expected from the behavior of each cell type separately. In heterozygous cultures at the higher population density, the incorporation of hypoxanthine relative to adenine is 28% greater than in those at the lower population density. A 1:1 mixture of normal and mutant cells exhibited a 20% increase in the relative incorporation of hypoxanthine over the same four fold increase in population density. These observations appear to provide a quantitative expression of the phenomenon of metabolic cooperation between contacting H\u2010GPRT\u2010negative and H\u2010GPRT\u2010positive cells.<\/jats:p>","DOI":"10.1002\/jcp.1040800105","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:58:29Z","timestamp":1109440709000},"page":"33-40","source":"Crossref","is-referenced-by-count":7,"title":["Lesch\u2010Nyhan mutation: The influence of population density on purine phosphoribosyltransferase activities and exogenous purine utilization in monolayer cultures of skin fibroblasts"],"prefix":"10.1002","volume":"80","author":[{"given":"Stephen","family":"Wood","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard","family":"Pinsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.169.3944.482"},{"key":"e_1_2_1_3_1","first-page":"596","article-title":"Detection of females heterozygous for the Lesch\u2010Nyhan mutation by 8\u2010azaguanine\u2010resistant growth of cultured fibroblasts","volume":"77","author":"Felix J. 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