{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T02:37:41Z","timestamp":1770431861462,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":10902,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1975,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A variant Chinese hamster cell line has been isolated from a mutagenized population that has a markedly reduced ability to oxidize a variety of substrates via the Krebs cycle. The production of <jats:sup>14<\/jats:sup>CO<jats:sub>2<\/jats:sub> from <jats:sup>14<\/jats:sup>C\u2010labeled compounds was measured using pyruvate, acetate, \u03b2\u2010hydroxybutyrate, palmitate and glutamate, and in all cases it was negligible in the mutant. In contrast to this, significant amounts of <jats:sup>14<\/jats:sup>CO<jats:sub>2<\/jats:sub> were produced from <jats:sup>14<\/jats:sup>C\u2010aspartate and <jats:sup>14<\/jats:sup>C\u2010succinate which suggests that some reactions of the Krebs cycle can take place and this conclusion is supported by tracer experiments with labeled compounds. The rate of respiration measured with a Clark oxygen electrode in the mutant was compared to several normal Chinese hamster cell lines and was found to be only 8%. Mitochondria appear to be present in normal numbers and with only minor differences in morphology. The measurement of difference spectra between oxidized and reduced states permits us to conclude that the cytochromes are all present and functional. These results lead us to believe that there may be a defect in the Krebs cycle between \u03b1\u2010ketoglutarate and succinate. Alternatively a defect in a structural component of the mitochondria or in the electron\u2010transport chain itself may be causing pleiotropic effects in the Krebs cycle and respiration.<\/jats:p>","DOI":"10.1002\/jcp.1040850216","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:53:10Z","timestamp":1109440390000},"page":"293-305","source":"Crossref","is-referenced-by-count":39,"title":["Conditionally lethal mutations in Chinese hamster cells. Characterization of a cell line with a possible defect in the krebs cycle"],"prefix":"10.1002","volume":"85","author":[{"given":"L.","family":"Defrancesco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Werntz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. E.","family":"Scheffler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1961.tb13503.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-106-26267"},{"key":"e_1_2_1_4_1","first-page":"6599","article-title":"Aspartate transcarbamylase. Interaction with the transition state analogue N(phosphonacetyl)\u2010L\u2010aspartate","volume":"246","author":"Collins K. 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