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ATP\u2010depleted wild type cells or thymidine kinase\u2010deficient sublines of Novikoff or L cells took up thymidine rapidly from the medium without concentrating it intracellularly, and exhibited countertransport of thymidine. Thus uptake was by facilitated diffusion. This transport system differs from the substrate\u2010specific, low\u2010K<jats:sub>m<\/jats:sub> (0.5 \u03bcM) thymidine transport system previously described for various types of cultured cells in that it exhibits an at least 100\u2010fold higher K<jats:sub>m<\/jats:sub> and transports equally well various ribo\u2010 and deoxyribonucleosides. The results suggest that the rate\u2010limiting step in thymidine incorporation into the nucleotide pool by wild type cells is phosphorylation rather than transport, or that the cells possess two transport systems, a facilitated diffusion system with low substrate specificity and a second system which involves substrate phosphorylation by thymidine kinase.<\/jats:p>","DOI":"10.1002\/jcp.1040890102","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:18:45Z","timestamp":1109438325000},"page":"1-18","source":"Crossref","is-referenced-by-count":71,"title":["Transport and countertransport of thymidine in ATP depleted and thymidine kinase\u2010deficient novikoff rat hepatoma and mouse L cells: Evidence for a high K<sub>m<\/sub> facilitated diffusion system with wide nucleoside specificity"],"prefix":"10.1002","volume":"89","author":[{"given":"Peter G. 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