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As demonstrated in several other cell types, ODC is inactivated very rapidly in HeLa cells, and the rate of inactivation is seen to vary with a half life of 9\u201315 minutes in uninduced cells and rises to <jats:italic>ca<\/jats:italic>. 60 minutes at the peak of induction. Quantitatively, the change in rate of inactivation cannot completely account for the observed rise in activity, thus synthesis or activation of ODC must also be involved in the induction process. The inactivation process requires metabolic energy and it can be sustained by glycolytic derived energy. Other factors which are known to inhibit protein breakdown in mammalian cells, such as sodium fluoride, insulin, or tosyl phenylalanyl chloromethyl ketone, had no effect on the rate of inactivation of ODC. Attempts to demonstrate ODC inactivation in a cell free system at neutral pH were unsuccessful.<\/jats:p>","DOI":"10.1002\/jcp.1040890107","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:18:45Z","timestamp":1109438325000},"page":"65-76","source":"Crossref","is-referenced-by-count":34,"title":["Ornithine decarboxylase inactivation in HeLa cells"],"prefix":"10.1002","volume":"89","author":[{"given":"W. 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