{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T13:40:37Z","timestamp":1699796437844},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":10292,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1976,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have shown that mutations in the <jats:italic>unc<\/jats:italic> gene of <jats:italic>Escherichia coli<\/jats:italic> K12 cause defects in energy transduction as well as in a membrane\u2010bound (Mg<jats:sup>2+<\/jats:sup>,Ca<jats:sup>2+<\/jats:sup>)\u00b7 adenosine triphosphatase.<\/jats:p><jats:p>We studied the effect of this mutation on the \u201cdownhill\u201d efflux of methyl\u2010\u03b2\u2010D\u2010galactopyranoside, a substrate of the <jats:italic>mgl<\/jats:italic> transport system. While <jats:italic>unc<\/jats:italic><jats:sup>+<\/jats:sup> and <jats:italic>unc<\/jats:italic><jats:sup>\u2212<\/jats:sup> isogenic strains of <jats:italic>E. coli<\/jats:italic> K12 did not show significant differences in substrate influx or efflux, a differential effect of an uncoupler, 2,4\u2010dinitrophenol was demonstrated. In contrast to the <jats:italic>unc<\/jats:italic><jats:sup>+<\/jats:sup>, dinitrophenol failed to inhibit significantly the rate coefficient of efflux in the <jats:italic>unc<\/jats:italic><jats:sup>\u2212<\/jats:sup> strain.<\/jats:p><jats:p>Analyses of spontaneous <jats:italic>unc<\/jats:italic><jats:sup>+<\/jats:sup> revertants of the <jats:italic>unc<\/jats:italic><jats:sup>\u2212<\/jats:sup> mutant provided additional evidence that a functional <jats:italic>unc<\/jats:italic> gene is necessary for dinitrophenol inhibition of efflux.<\/jats:p><jats:p>Other uncouplers tested in the <jats:italic>unc<\/jats:italic><jats:sup>+<\/jats:sup> strain showed different effects on efflux. While arsenate, azide and carbonyl cyanide p\u2010trifluoromethoxyphenylhydrazone caused little or no effect, 2,4\u2010dibromophenol and pentachlorophenol increased efflux by a considerable factor.<\/jats:p>","DOI":"10.1002\/jcp.1040890411","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:28:41Z","timestamp":1109438921000},"page":"561-566","source":"Crossref","is-referenced-by-count":1,"title":["Effect of uncoupler on \u201cDownhill\u201d substrate efflux of <i>Escherichia coli<\/i> is dependent on (Mg<sup>2+<\/sup>,Ca<sup>2+<\/sup>)\u00b7 adenosine triphosphatase"],"prefix":"10.1002","volume":"89","author":[{"given":"Boris","family":"Rotman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"7747","DOI":"10.1016\/S0021-9258(19)42031-0","article-title":"Different mechanisms of energy coupling for the shock\u2010sensitive and shock\u2010resistant amino acid permeases of Escherichia coli","volume":"249","author":"Berger E. A.","year":"1974","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.43.070174.001011"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1240075"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1128\/jb.123.1.187-195.1975","article-title":"Effect of uncouplers on \u201cdownhill\u201d \u03b2\u2010galactoside transport in energy\u2010depleted cells of Escherichia coli","volume":"123","author":"Cecchini G.","year":"1975","journal-title":"J. Bacteriol."},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1515\/znc-1974-1-214","article-title":"The membrane ATPase of the (Mg, Ca)\u2010ATPase\u2010mutant E. coli K12, strain AN120","volume":"29","author":"Gunther T.","year":"1974","journal-title":"Z. Naturforsch. C."},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1016\/S0021-9258(19)76845-8","article-title":"Galactose transport in Escherichia coli. II. Characteristics of the exit process","volume":"235","author":"Horecker B. L.","year":"1960","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.1016\/S0021-9258(19)61703-5","article-title":"Galactose transport in Escherichia coli. III. The effect of 2,4\u2010dinitrophenol on entry and accumulation","volume":"236","author":"Osborn M. J.","year":"1961","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"4436","DOI":"10.1016\/S0021-9258(19)43788-5","article-title":"Unidirectional transport activity mediated by the galactose\u2010binding protein of Escherichia coli","volume":"248","author":"Parnes J. R.","year":"1973","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1128\/jb.123.1.69-74.1975","article-title":"Regulation of the Escherichia coli methylgalactoside transport system by gene mglD.","volume":"123","author":"Robbins A. R.","year":"1975","journal-title":"J. Bacteriol."},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.2.423"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.1016\/S0021-9258(17)33505-6","article-title":"Roles of individual mgl gene products in the methylgalactoside transport system of Escherichia coli K12","volume":"251","author":"Robbins A. R.","year":"1976","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(68)90379-3"},{"key":"e_1_2_1_14_1","first-page":"806","article-title":"Transport of galactose from the inside to the outside of Escherichia coli","volume":"9","author":"Rotman B.","year":"1961","journal-title":"Pathol. Biol. Semaine Hop."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.44.070175.002515"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1128\/br.36.4.504-524.1972","article-title":"Linkage map of Escherichia coli strain K\u201012","volume":"36","author":"Taylor A. L.","year":"1972","journal-title":"Bacteriol. Rev."}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1040890411","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1040890411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T13:16:29Z","timestamp":1699794989000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1040890411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,12]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1976,12]]}},"alternative-id":["10.1002\/jcp.1040890411"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1040890411","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,12]]}}}