{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T21:38:55Z","timestamp":1766266735654},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":10292,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1976,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Techniques are described by which the transport of nutrients into mammalian cells in suspension can be measured at intervals of 1.5 seconds. By application of these techniques, the existence of a saturable (K<jats:sub>m<\/jats:sub> = 85 \u03bcM), non\u2010concentrative, transport system for thymidine was demonstrated in Novikoff rat hepatoma cells depleted of ATP. At concentrations of thymidine less than the K<jats:sub>m<\/jats:sub>, this system operated at velocities sufficient to nearly completely equilibrate intra\u2010 and extra\u2010cellular thymidine pools within 8 seconds. In phosphorylating cells, the transport system operated with similar rapidity, so that intracellular phosphorylation was rate\u2010limiting for the incorporation of thymidine into nucleotides. Uptake of 3\u2010O\u2010methylglucose occurred at comparable velocities, attaining 90% of equilibrium between internal and external pools within 25 seconds. Uptake of cytosine by simple diffusion was 100 times slower.<\/jats:p>","DOI":"10.1002\/jcp.1040890417","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:28:41Z","timestamp":1109438921000},"page":"605-612","source":"Crossref","is-referenced-by-count":80,"title":["The application of rapid kinetic techniques to the transport of thymidine and 3\u2010O\u2010methylglucose into mammalian cells in suspension culture"],"prefix":"10.1002","volume":"89","author":[{"given":"Robert M.","family":"Wohlhueter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Marz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon C.","family":"Graff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter G. 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