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Uptake of valine by hepatocytes from 1.5\u2010 and 18\u2010month\u2010old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1\u2010 to 2\u2010month\u2010old rats is 96.8 pmoles of valine per minute per milligram protein. 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