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This effect was observed in two sublines examined in this study, HeLa<jats:sub>65<\/jats:sub> and HeLa<jats:sub>71<\/jats:sub>. hCG and FSH were measured by radioimmunoassay using antiserum against the \u03b2\u2010subunit of the hormone dimer, thus insuring immunochemical specificity. The amounts of hCG and FSH produced by HeLa<jats:sub>65<\/jats:sub> and HeLa<jats:sub>71<\/jats:sub> cells cultured in hyperosmolar medium were 2\u2010 to 50\u2010fold higher than corresponding hormone levels in basal cultures. Synthesis of gonadotropins depended on concentration and duration of exposure to hyperosmolar medium. Levels of culture medium osmolality effective in inducing hormone production also inhibit the incorporation of <jats:sup>14<\/jats:sup>C\u2010thymidine into acid\u2010insoluble macromolecules. Hyperosmolality thus stimulates the ectopic production of gonadotropic hormones while retarding cellular growth and nucleic acid synthesis.<\/jats:p>","DOI":"10.1002\/jcp.1040980319","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T16:51:25Z","timestamp":1109436685000},"page":"613-618","source":"Crossref","is-referenced-by-count":5,"title":["Induction of human choriogonadotropin and follitropin in HeLa cell cultures by hyperosmolality"],"prefix":"10.1002","volume":"98","author":[{"given":"Robert J.","family":"Fallon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rody P.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nimai K.","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(77)90069-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/259416a0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/267435a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1660265"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0880137"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-149-38857"},{"key":"e_1_2_1_8_1","first-page":"1943","article-title":"Modulation of alkaline phosphatase activity in cell cultures derived from human urinary bladder carcinoma","volume":"34","author":"Herz F.","year":"1974","journal-title":"Can. 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