{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T02:21:52Z","timestamp":1771640512402,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":8741,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1981,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have demonstrated that serum contains mitogens, such as platelet\u2010derived growth factor (PDGF), which may alter fibroblast responsiveness to growth factors contained in plasma. Somatomedin\u2010C (SM\u2010C) has been identified as one of the plasma growth factors required for mouse Balb\/c 3T3 fibroblasts to initiate DNA synthesis. The present experiments were undertaken to explore the interaction between PDGF, human growth hormone (hGH), SM\u2010C, and other growth\u2010promoting agents in stimulating the growth of human fibroblasts. Proliferation of human dermal fibroblasts plated at low density (3,000 cells\/cm<jats:sup>2<\/jats:sup>) was found to be equally stimulated by continuous exposure to either normal or somatomedin\u2010C\u2010deficient serum. In contrast, when confluent monolayers were sequentially exposed to PDGF, followed either by normal platelet poor plasma (PPP) or hypopituitary PPP, the cells exposed to normal PPP entered the \u201cS\u201d phase of the cell cycle 50% faster. This difference could be abolished by a 6\u2010hour incubation with growth hormone (10 ng\/ml) or somatomedin\u2010C (5 ng\/ml) preceding the addition of plasma. When medium containing either hGH or Sm\u2010C was changed frequently so as to remove factors secreted by fibroblasts, only those cells exposed to exogenous somatomedin\u2010C entered DNA synthesis. This finding is in agreement with previous findings that human fibroblasts are capable of making Sm\u2010C in response to hGH. These findings support the hypothesis that somatomedin is required for fibroblast replication in vitro, and that growth hormone appears to stimulate replication indirectly through somatomedin production.<\/jats:p>","DOI":"10.1002\/jcp.1041060305","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T16:11:48Z","timestamp":1109434308000},"page":"361-367","source":"Crossref","is-referenced-by-count":72,"title":["Somatomedin\u2010C and platelet\u2010derived growth factor stimulate human fibroblast replication"],"prefix":"10.1002","volume":"106","author":[{"given":"David R.","family":"Clemmons","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judson J.","family":"Van Wyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.7.2635"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.4.1809"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0028-1093939"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.2.369"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(77)90089-1"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110001"},{"key":"e_1_2_1_8_1","first-page":"357","article-title":"Synchronous activation of DNA synthesis in hypophysectomized rat cartilage by growth hormone","volume":"68","author":"Daughaday W. H.","year":"1966","journal-title":"J. Lab. Clin. Med."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/272356a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040810205"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108816"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/263140a0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(76)90477-8"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.7.2908"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"2679","DOI":"10.1016\/S0021-9258(17)40874-X","article-title":"Anatomically occurring structural variant of human growth hormone","volume":"253","author":"Lewis U. J.","year":"1978","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-40-1-62"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(78)91346-3"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040930104"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-46-6-937"},{"key":"e_1_2_1_20_1","unstructured":"Nissley S. P. andM. M.Rechler(1977)Multiplication stimulating activity (MSA): A somatomedin\u2010like polypeptide from cultured rat liver cells. In: Third Dicennial Review Conference: Cell Tissue and Organ Cultures NCI monograph # 48 pp.167\u2013178."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.10.4481"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.6.2839"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-39-3-512"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.69.1.196"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.6.1455"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.4.1263"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.3.1279"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040780304"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00545a027"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-452805-5.50009-0"},{"key":"e_1_2_1_31_1","unstructured":"Weidman E. R. P.Atkinson andR. M.Bala(1979)Production of somatomedin and mitogenic effects of somatomedin on human fibroblasts. Presented at the 61st meeting of the Endocrine Society Anaheim California June 13\u201315."},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(78)90267-6"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041060305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041060305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T13:50:11Z","timestamp":1697723411000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041060305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,3]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1981,3]]}},"alternative-id":["10.1002\/jcp.1041060305"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041060305","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,3]]}}}