{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T21:38:35Z","timestamp":1766266715377},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":8588,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1981,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inhibition of the gap\u2010filling, polymerizing step of excision repair by 1\u2010\u03b2\u2010D\u2010arabinofuranosylcytosine (ara\u2010C) after irradiation with ultraviolet light in human diploid fibroblasts resulted in the formation of persistent DNA strand breaks in G<jats:sub>1<\/jats:sub>, G<jats:sub>2<\/jats:sub>, and plateau phase cells, but not in S phase cells. Addition of hydroxyurea to ara\u2010C resulted in partial inhibition of repair in S phase cells. These observations can be explained either in terms of changing roles in repair for different DNA polymerases throughout the cell cycle or by the presence of a pool of deoxycytidine nucleotides during S phase equivalent to an external source of deoxycytidine at 50 \u03bcM concentration. A similar concentration dependence on ara\u2010C was observed for inhibition of repair in normal human, xeroderma pigmentosum (XP) variant, and Cockayne's syndrome cells. Ara\u2010C produced a similar number of breaks in normal and Cockayne's syndrome cells but slightly more in XP variant cells. Exonuclease III and S1 nuclease independently both degraded about 50% of the <jats:sub>3<\/jats:sub>H\u2010thymidine incorporated into repaired regions in the presence of ara\u2010C. Sequential digestion with both enzymes degraded nearly 90% of the repaired regions. These observations can be explained if excision repair proceeds by displacing the damaged strand so that both the <jats:sub>3<\/jats:sub>H\u2010labeled patch and the damaged region are still ligated to high molecular weight DNA and compete for the same complementary strand during in vitro incubation with the nucleases. The amount of <jats:sub>3<\/jats:sub>H\u2010thymidine incorporated in DNA by repair decreased with increasing concentrations of ara\u2010C and hydroxyurea, suggesting that the incomplete patches became shorter under these conditions. Extrapolation of the digestion kinetics with exonuclease III permits an estimate of the normal patch size of about 100 nucleotides, consistent with previous estimates.<\/jats:p>","DOI":"10.1002\/jcp.1041080207","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T15:46:46Z","timestamp":1109432806000},"page":"163-173","source":"Crossref","is-referenced-by-count":40,"title":["Sensitivity of excision repair in normal human, xeroderma pigmentosum variant and Cockayne's syndrome fibroblasts to inhibition by cytosine arabinoside"],"prefix":"10.1002","volume":"108","author":[{"given":"James E.","family":"Cleaver","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(79)90966-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.2.794"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(69)86373-3"},{"key":"e_1_2_1_5_1","first-page":"297","volume-title":"Introduction of Macromolecules into Viable Mammalian Cells","author":"Castellot J. J.","year":"1980"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.5.1205"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(78)90148-3"},{"key":"e_1_2_1_8_1","volume-title":"Thymidine Metabolism and Cell Kinetics","author":"Cleaver J. E.","year":"1967"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.2307\/3572737"},{"key":"e_1_2_1_10_1","first-page":"1","volume-title":"Advances in Radiation Biology","author":"Cleaver J. E.","year":"1974"},{"key":"e_1_2_1_11_1","first-page":"123","volume-title":"Methods in Cancer Research","author":"Cleaver J. E.","year":"1975"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/270451a0"},{"key":"e_1_2_1_13_1","first-page":"489","article-title":"DNA repair and its coupling to DNA replication in eukaryotic cells","volume":"516","author":"Cleaver J. E.","year":"1978","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/1.8.647"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(65)90086-9"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(79)90193-X"},{"key":"e_1_2_1_17_1","first-page":"3621","article-title":"Selective response of DNA polymerase \u03b2 to bleomycin\u2010induced breaks in DNA","volume":"38","author":"Coetzee M. L.","year":"1978","journal-title":"Cancer Res."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041030203"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(78)90256-3"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.46.070177.003233"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553007614550801"},{"key":"e_1_2_1_22_1","first-page":"367","article-title":"Inhibition of DNA excision repair in human cells by arabinofuranosyl cytosine: Effect on normal and xeroderma pigmentosum cells","volume":"15","author":"Dunn W. C.","year":"1979","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(78)85487-3"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"6128","DOI":"10.1016\/S0021-9258(18)50528-7","article-title":"En\u2010zymological characterization of DNA polymerase \u03b1. Basic catalytic properties, processivity, and gap utilization of the homogeneous enzyme from human KB cells","volume":"254","author":"Fisher P. A.","year":"1979","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.1.39"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(79)90057-1"},{"key":"e_1_2_1_27_1","unstructured":"Fujiwara Y. andY.Satoh(1980) Xeroderma pigmentosum variant: Molecular defect caffeine susceptibility and cancer.J. Invest. Dermatol.(in press)."},{"key":"e_1_2_1_28_1","first-page":"1591","article-title":"An increase in DNA polymerase \u03b2 in rat liver nuclei in response to partial hepatectomy (abstract)","volume":"35","author":"Gerwin B. I.","year":"1976","journal-title":"Fed. Proc."},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.48.070179.004031"},{"key":"e_1_2_1_30_1","first-page":"13","article-title":"Clinical characteristics, DNA repair, and complementation groups in xeroderma pigmentosum patients from Egypt","volume":"40","author":"Hashem N.","year":"1980","journal-title":"Cancer Res."},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(77)90238-6"},{"key":"e_1_2_1_32_1","first-page":"347","article-title":"Pigmented xerodermoid: First report of a family","volume":"65","author":"Hofmann H.","year":"1978","journal-title":"Bull. Cancer"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.5.2316"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(80)85104-6"},{"key":"e_1_2_1_35_1","unstructured":"Kaufmann W. K. andJ. E.Cleaver(1980) Inhibition of replicon initiation and DNA chain elongation by ultraviolet light in normal human and xeroderma pigmentosum fibroblasts.J. Mol. Biol.(in press)."},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(80)90147-1"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/6.1.331"},{"key":"e_1_2_1_38_1","first-page":"4237","article-title":"Abnormal kinetics of DNA synthesis in ultraviolet light\u2010irradiated cells from patients with Cockayne's syndrome","volume":"39","author":"Lehmann A. R.","year":"1979","journal-title":"Cancer Res."},{"key":"e_1_2_1_39_1","first-page":"901","article-title":"The 7. S nuclear DNA polymerase and DNA replication in intact liver","volume":"36","author":"Lynch W. E.","year":"1976","journal-title":"Cancer Res."},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.8.3927"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(80)90034-9"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(74)90819-2"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)38226-1"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00550a009"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(79)90462-5"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/1.1.9"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(75)90017-3"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(78)85488-5"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(79)90126-6"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041080207","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041080207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T08:16:09Z","timestamp":1699776969000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041080207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,8]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1981,8]]}},"alternative-id":["10.1002\/jcp.1041080207"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041080207","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,8]]}}}