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Differentiation of NB\u201015 cells was induced by the addition of dibutyryl cyclic AMP and 3\u2010isobutyl\u20101\u2010methylxanthin (IBMX) to the growth medium and was monitored by neurite outgrowth, increase of acetylcholinesterase (AChE), and R<jats:sub>I<\/jats:sub> cAMP\u2010binding protein. Plating of NB\u201015 cells in fresh serum\u2010containing growth medium was accompanied by rapid growth and a marked increase of ODC activity, this early increase of ODC activity was attenuated, both in duration and in magnitude, in the differentiating cells. The spermidine content of the differentiating neuroblastoma cell was significantly lower than that of the nondifferentiating cells. In the fully differentiated neuroblastoma cells, the ODC activity and spermidine content were lower than that of the undifferentiated cells by approximately 15\u2010fold and five\u2010fold, respectively. Based on these results it is proposed that changes of polyamine metabolism may be of significance in the differentiation of mouse neuroblastoma cells.<\/jats:p>","DOI":"10.1002\/jcp.1041100311","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T15:15:38Z","timestamp":1109430938000},"page":"285-290","source":"Crossref","is-referenced-by-count":28,"title":["Changes of ornithine decarboxylase activity and polyamine content upon differentiation of mouse NB\u201015 neuroblastoma cells"],"prefix":"10.1002","volume":"110","author":[{"given":"Kuang Yu","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Presepe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Parken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice Y.\u2010C.","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"1","volume-title":"Function of Naturally Occuring Polyamines","author":"Bachrach U.","year":"1973"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.8.3087"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(72)90904-7"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(77)90085-X"},{"key":"e_1_2_1_6_1","first-page":"241","article-title":"Induction of ornithine decarboxylase in Reuber H35 rat hepatoma cells","volume":"2","author":"Byus C. 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