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By applying an autoradiographic technique to visualize transported alanine, it is clear, however, that even in subconfluent cultures there is marked cellular inhomogeneity with regard to uptake, which takes place predominantly in those cells at the periphery of growing islands and not those at the interior. In contrast, when cells are grown on permeable substrata, a uniform distribution of silver grains is found. In two other types of experiment, we found that when confluent cell monolayers on an impermeable support were treated briefly with a chelating agent or suspended by mechanical treatment, there was a marked increase per cell in sodium\u2010dependent alanine uptake and in ouabain\u2010sensitive potassium uptake. We conclude that the apparent fall in alanine uptake as cells reach confluence on an impermeable support is due to masking of transport sites, which are predominantly, if not exclusively, located at the basolateral membrane.<\/jats:p>","DOI":"10.1002\/jcp.1041180214","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T14:51:49Z","timestamp":1109429509000},"page":"211-217","source":"Crossref","is-referenced-by-count":23,"title":["Localisation of Alanine uptake by cultured renal epithelial cells (LLC\u2010PK<sub>1<\/sub>) to the basolateral membrane"],"prefix":"10.1002","volume":"118","author":[{"given":"Francisco V.","family":"Sepulveda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy D.","family":"Pearson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1982.242.1.C94"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041130209"},{"key":"e_1_2_1_4_1","first-page":"257","volume-title":"Membranes and Ion Transport","author":"Bonting S. 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