{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:00:06Z","timestamp":1758279606396},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":7614,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1984,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The anion requirement for toxic action of diphtheria toxin and modeccin was studied. In Cl<jats:sup>\u2212<\/jats:sup>\u2010free Hepes buffer made isotonic with mannitol, cells were insensitive to diphtheria toxin and modeccin. Just 2 mM NaCl was sufficient to obtain full toxic activity of modeccin, whereas 140 mM NaCl was required for maximal intoxication with diphtheria toxin. Br<jats:sup>\u2212<\/jats:sup> could substitute for Cl<jats:sup>\u2212<\/jats:sup>. NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>, I<jats:sup>\u2212<\/jats:sup> and ClO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> were less efficient than Cl<jats:sup>\u2212<\/jats:sup>, whereas SO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup> and SCN<jats:sup>\u2212<\/jats:sup> were unable to replace Cl<jats:sup>\u2212<\/jats:sup>. Cl<jats:sup>\u2212<\/jats:sup> deprivation both reduced the ability of cells to bind diphtheria toxin and prevented bound toxin from intoxicating the cells. The binding of modeccin was not reduced. SITS (4\u2010acetamide\u20104\u2032\u2010isothio\u2010cyano\u2010stilbene\u20102,2\u2032\u2010disulfonic acid), an inhibitor of Cl<jats:sup>\u2212<\/jats:sup> entry, protected against diphtheria toxin and modeccin, indicating that Cl<jats:sup>\u2212<\/jats:sup> transport is required for intoxication.<\/jats:p>","DOI":"10.1002\/jcp.1041190103","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T14:04:09Z","timestamp":1109426649000},"page":"7-14","source":"Crossref","is-referenced-by-count":19,"title":["Anion requirement and effect of anion transport inhibitors on the response of vero cells to diphtheria toxin and modeccin"],"prefix":"10.1002","volume":"119","author":[{"given":"Kirsten","family":"Sandvig","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sjur","family":"Olsnes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.7.3562"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(78)90016-3"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"6866","DOI":"10.1016\/S0021-9258(17)38000-6","article-title":"Demonstration of diphtheria toxin receptors on surface membranes from both toxin sensitive and toxin resistant species","volume":"253","author":"Chang T.","year":"1978","journal-title":"J. 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