{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:22:19Z","timestamp":1757704939285},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":7461,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1984,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cyclic AMP phosphodiesterase (PDE) activity was characterized in culture of ewe myometrial cells and its sensitivity to steroid hormones was tested. Cultured myometrial cells were maintained from the first to the 20th subculture in the presence of 2% of serum in a medium supplemented with 1 \u03bcM of insulin. It was found that myometrial cells possess a PDE activity with atypical kinetics. The nonlinear responses in Lineweaver\u2010Burke plots suggest the presence of high\u2010 and low\u2010affinity PDE activities. In cell culture, apparent Km values were similar to those obtained from the original myometrium. Vmax values increased with successive subcultures, revealing an increase in the capacity of the cells to degrade cAMP; in parallel, the growth rate decreased. The PDE specific activity in cultured myometrial cells was inhibited by estra\u2010diol or progesterone. When added together, no synergistic effect was obtained. The rate of inhibition for both steroids was constant during successive passages for both low\u2010 and high\u2010affinity conditions. Results obtained in myometrial cell long\u2010term culture were compatible with reports in other species in vivo. Considering the role of cAMP in the regulation of uterine functions, subcultured myometrial cells provided us a useful experimental system with which to study the cAMP metabolism process.<\/jats:p>","DOI":"10.1002\/jcp.1041200318","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T14:16:18Z","timestamp":1109427378000},"page":"391-396","source":"Crossref","is-referenced-by-count":8,"title":["Evolution of cAMP phosphodiesterase activity in cultured myometrial cells: Effects of steroids and of successive subcultures"],"prefix":"10.1002","volume":"120","author":[{"given":"C.","family":"Vallet\u2010Strouve","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Ferre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Breuiller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(76)90071-X"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod21.2.309"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(76)90071-X"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0620315"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)70943-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0600369"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(75)80829-5"},{"key":"e_1_2_1_9_1","first-page":"73","article-title":"Activit\u00e9 3\u20325\u2032 AMP cyclique phosphodiesterase dans les couches externe et interne du myom\u00e8tre humain en fin de grossesse","volume":"285","author":"Ferre F.","year":"1977","journal-title":"C. R. Acad. Sci. [D], Paris"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(78)90468-9"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00608a009"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/292253a0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(80)90198-9"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod16.1.1"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(76)90111-5"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(78)90669-0"},{"key":"e_1_2_1_17_1","first-page":"328","article-title":"Changes in specific activities of 3\u2032,5\u2032 cyclic adenosine monophosphate phosphodiesterase in chick embryo cells grown under different conditions","volume":"26","author":"Krizanova O.","year":"1982","journal-title":"Acta. Virol."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(80)90246-9"},{"key":"e_1_2_1_19_1","volume-title":"Advances in Cyclic Nucleotide Research","author":"Leroy M. J."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107096"},{"key":"e_1_2_1_22_1","first-page":"671","article-title":"Stimulation of microsomal calcium uptake and protein phosphorylation of adeno sine cyclic 3\u2032,5\u2032\u2010monophosphate in rat uterus","volume":"131","author":"Nishikori K.","year":"1977","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/9780470110324.ch5","volume-title":"The determination of nucleic acids in methods of biochemical analysis XIV Glick D","author":"Munro H. N.","year":"1966"},{"key":"e_1_2_1_24_1","first-page":"252","article-title":"Effects of growth conditions on cyclic nucleotide phosphodiesterase of cultured fibrolasts","volume":"1","author":"Pledger W. J.","year":"1975","journal-title":"J. Cyclic Nucleotide Rcs."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041000312"},{"key":"e_1_2_1_26_1","first-page":"52","article-title":"Regulation of myometrial adrenoreceptors and adrenergic response to sex steroids","volume":"20","author":"Roberts J. M.","year":"1981","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_1_27_1","first-page":"3","article-title":"Association between myosin light chain phosphorylation and contraction of rat uterine smooth muscle","volume":"1","author":"Sanis R. A.","year":"1981","journal-title":"Mol. Physiol."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/277032a0"},{"key":"e_1_2_1_29_1","doi-asserted-by":"crossref","first-page":"9951","DOI":"10.1016\/S0021-9258(19)86651-6","article-title":"Adenosine 3\u2032:5\u2010monophosphate\u2010mediated inhibition of myosine light chain phosphorylation in bovine aortic actomyosin","volume":"254","author":"Silver P. J.","year":"1979","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90327-5"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(75)90010-6"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00778a018"},{"key":"e_1_2_1_33_1","first-page":"15","article-title":"Myometrial cells in primary culture: Characterization and hormonal profile","volume":"8","author":"Vallet\u2010strouve C.","year":"1978","journal-title":"Mol. Cell. Endocrinol."},{"key":"e_1_2_1_34_1","first-page":"1","article-title":"Culture de cellules myometriales; un modele pour l'etude de l'action de I'oestradiol","volume":"39","author":"Vallet\u2010strouve C.","year":"1980","journal-title":"Biol. Cell."},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(82)90598-2"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod19.2.318"},{"key":"e_1_2_1_37_1","first-page":"263","volume-title":"Advances in Cyclic Nucleotide Research","author":"Vaughan M.","year":"1980"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041200318","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041200318","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T08:47:35Z","timestamp":1697791655000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041200318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,9]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1984,9]]}},"alternative-id":["10.1002\/jcp.1041200318"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041200318","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,9]]}}}